Biofabrication of in Situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated Using Magnetic Levitation

Overview

Journal Sci Rep

Specialty Science

Date 2018 May 10

PMID 29740095

Citations 33

Authors

Muge Anil-Inevi

Sena Yaman

Ahu Arslan Yildiz

Gulistan Mese

Ozden Yalcin-Ozuysal

H Cumhur Tekin

Engin Ozcivici

Affiliations

Soon will be listed here.

Abstract

Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material and biological sciences. Yet little is known about the ability of the magnetic levitation system to facilitate biofabrication of in situ three dimensional (3D) cellular structures. Here, we optimized a magnetic levitation though negative magnetophoresis protocol appropriate for long term levitated cell culture and developed an in situ 3D cellular assembly model with controlled cluster size and cellular pattern under simulated weightlessness. The developed strategy outlines a potential basis for the study of weightlessness on 3D living structures and with the opportunity for real-time imaging that is not possible with current ground-based simulated weightlessness techniques. The low-cost technique presented here may offer a wide range of biomedical applications in several research fields, including mechanobiology, drug discovery and developmental biology.

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