Effect of Herbal Extracts on Bone Regeneration in a Rat Calvaria Defect Model and Screening System

Overview

Journal J Korean Assoc Oral Maxillofac Surg

Date 2018 May 8

PMID 29732313

Citations 5

Authors

Dong-Hwan Lee

Il-Kyu Kim

Hyun-Young Cho

Ji-Hoon Seo

Jun-Min Jang

Jin Kim

Affiliations

Soon will be listed here.

Abstract

Objectives: The aim of this study was to evaluate the effects of herbal extracts on bone regeneration. Two known samples were screened.

Materials And Methods: We previously established a rat calvaria defect model using a combination of collagen scaffold and herbal extracts. An 8 mm diameter trephine bur with a low-speed dental hand piece was used to create a circular calvaria defect. The experimental group was divided into 4 classifications: control, collagen matrix, Danshen with collagen, and Ge Gan with collagen. Animals in each group were sacrificed at 4, 6, 8, and 10 weeks after surgery, and bone regeneration ability was evaluated by histological examination.

Results: Results revealed that both Danshen and Ge Gan extracts increased bone formation activity when used with collagen matrix. All groups showed almost the same histological findings until 6 weeks. However, after 6 weeks, bone formation activity proceeded differently in each group. In the experimental groups, new bone formation activity was found continuously up to 10 weeks. In the Danshen and Ge Gan groups, grafted materials were still present until 10 weeks after treatment, as evidenced by foreign body reactions showing multinucleated giant cells in chronic inflammatory vascular connective tissue.

Conclusion: Histological analyses showed that Danshen and Ge Gan extractions increased bone formation activity when used in conjunction with collagen matrix.

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