Effects of DKK1 Overexpression on Bone Metastasis of SBC-3 Cells

Overview

Journal Oncol Lett

Specialty Oncology

Date 2018 May 8

PMID 29731859

Citations 10

Authors

Hailin Pang

Ningqiang Ma

Weiwei Shen

Qiang Zhao

Jianlin Wang

Lian Duan

Wenjuan Chen

Ning Zhang

Zhengwei Zhao

Lili Liu

Helong Zhang

Affiliations

Soon will be listed here.

Abstract

Among all malignancies, lung cancer is the leading cause of cancer-related deaths in China. Bone metastasis is one of the most common complications and one of the most important factors affecting the prognosis of lung cancer patients, which resulting in very poor therapeutic effects. Previously, we have demonstrated that the expression levels of Dickkopf1 (DKK1), a protein involved in cell regulation and proliferation, was dramatically higher in cells that have a tendency to metastasize and invade the bone tissue (SBC-5 cells) compared with cells that do not (SBC-3 cells). Downregulation of DKK1 in SBC-5 cells inhibited cell malignancy , and the formation of bone metastasis . However, whether upregulating DKK1 would be sufficient to induce aggressive tumor behavior (proliferation, migration, invasion and metastasis) in SBC-3 cells remained to be investigated. The present study aimed to examine the role of DKK1 in SBC-3 cells, as well as to investigate the SBC-3 ability to metastasize and invade the bone tissue. The results demonstrated that upregulation of DKK1 in SBC-3 cells enhanced cell proliferation, colony formation, cell migration and invasion , as well as bone metastasis . These results indicate that DKK1 may be an important regulator in the development of small cell lung cancer (SCLC), and targeting DKK1 may be an effective method for preventing and/or treating skeletal metastases in SCLC cases.

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