The Effect of Mast Cells on the Biological Characteristics of Prostate Cancer Cells

Overview

Journal Cent Eur J Immunol

Publisher Termedia

Specialty Allergy & Immunology

Date 2018 May 8

PMID 29731687

Citations 4

Authors

Zhifang Ma

Liang Yue

Zhaoliang Xu

Sheng Zeng

Yukun Ma

Zhuoping Li

Wei Li

Dongwen Wang

Affiliations

Soon will be listed here.

Abstract

Aim Of The Study: To investigate the effects of mast cells on the proliferation, invasion, and metastasis of prostate cancer cells.

Material And Methods: The mast cell P815 and prostate cancer LNCaP cells were chosen using a Transwell chamber to construct a two-cell cocultured in vitro model to observe the migration of mast cells to prostate cancer cells.

Results: In the migration experiment, the migration rate of mast cells from the experimental group (%) was 10.167 0.833, the mast cell migration rate (%) of the control group was 0.833 0.208, and the difference was statistically significant (p < 0.05). The MTT test showed that the OD value of cells in each group over time increased gradually, and 24 h after LNCaP cells were cocultured with different concentrations of mast cells, the OD value was significantly higher than that of the control group (p < 0.05). QRT-PCR and western blot results showed that, compared with the control group, E-cad expression from the experimental group was significantly weakened; N-cad and vimentin expression increased (p < 0.05), and c-kit and SCF expression from experimental group were significantly higher than that of the control group (p < 0.05). After the addition of c-kit neutralising antibodies, compared with the control group, the mast cell migration rate of experimental group decreased significantly and prostate cancer cell proliferation significantly decreased (p < 0.05).

Conclusions: Mast cells could promote the proliferation of prostate cancer cells and the occurrence of epithelial mesenchymal transition (EMT), which could promote the invasion and metastasis of prostate cancer cells.

Citing Articles

The regulatory role and mechanism of mast cells in tumor microenvironment.

Ligan C, Ma X, Zhao S, Zhao W Am J Cancer Res. 2024; 14(1):1-15.

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Fernandes R, Costa C, Fernandes R, Barros A Biomedicines. 2023; 11(12).

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Suppression of TRPM7 Inhibited Hypoxia-Induced Migration and Invasion of Androgen-Independent Prostate Cancer Cells by Enhancing RACK1-Mediated Degradation of HIF-1.

Yang F, Cai J, Zhan H, Situ J, Li W, Mao Y Oxid Med Cell Longev. 2020; 2020:6724810.

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References

Johansson A, Rudolfsson S, Hammarsten P, Halin S, Pietras K, Jones J . Mast cells are novel independent prognostic markers in prostate cancer and represent a target for therapy. Am J Pathol. 2010; 177(2):1031-41. PMC: 2913352. DOI: 10.2353/ajpath.2010.100070. View

Knapczyk-Stwora K, Grzesiak M, Duda M, Koziorowski M, Slomczynska M . Effect of flutamide on folliculogenesis in the fetal porcine ovary--regulation by Kit ligand/c-Kit and IGF1/IGF1R systems. Anim Reprod Sci. 2013; 142(3-4):160-7. DOI: 10.1016/j.anireprosci.2013.09.014. View

Craig M, Ying C, Loberg R . Co-inoculation of prostate cancer cells with U937 enhances tumor growth and angiogenesis in vivo. J Cell Biochem. 2007; 103(1):1-8. DOI: 10.1002/jcb.21379. View

Gupta S, Takebe N, LoRusso P . Targeting the Hedgehog pathway in cancer. Ther Adv Med Oncol. 2011; 2(4):237-50. PMC: 3126020. DOI: 10.1177/1758834010366430. View

Klein E, Silverman R . Inflammation, infection, and prostate cancer. Curr Opin Urol. 2008; 18(3):315-9. DOI: 10.1097/MOU.0b013e3282f9b3b7. View

Moon T, St Laurent C, Morris K, Marcet C, Yoshimura T, Sekar Y . Advances in mast cell biology: new understanding of heterogeneity and function. Mucosal Immunol. 2010; 3(2):111-28. DOI: 10.1038/mi.2009.136. View

Figueira M, Correia S, Vaz C, Cardoso H, Gomes I, Marques R . Estrogens down-regulate the stem cell factor (SCF)/c-KIT system in prostate cells: Evidence of antiproliferative and proapoptotic effects. Biochem Pharmacol. 2015; 99:73-87. DOI: 10.1016/j.bcp.2015.11.016. View

Jemal A, Siegel R, Xu J, Ward E . Cancer statistics, 2010. CA Cancer J Clin. 2010; 60(5):277-300. DOI: 10.3322/caac.20073. View

Daniels N, Ewing S, Zmuda J, Wilt T, Bauer D . Correlates and prevalence of prostatitis in a large community-based cohort of older men. Urology. 2005; 66(5):964-70. DOI: 10.1016/j.urology.2005.05.034. View

10.

Hsu A, Bray T, Ho E . Anti-inflammatory activity of soy and tea in prostate cancer prevention. Exp Biol Med (Maywood). 2010; 235(6):659-67. PMC: 4125123. DOI: 10.1258/ebm.2010.009335. View

11.

Coussens L, Werb Z . Inflammation and cancer. Nature. 2002; 420(6917):860-7. PMC: 2803035. DOI: 10.1038/nature01322. View

12.

McDonald P, Chafe S, Dedhar S . Overcoming Hypoxia-Mediated Tumor Progression: Combinatorial Approaches Targeting pH Regulation, Angiogenesis and Immune Dysfunction. Front Cell Dev Biol. 2016; 4:27. PMC: 4814851. DOI: 10.3389/fcell.2016.00027. View

13.

Clevers H . At the crossroads of inflammation and cancer. Cell. 2004; 118(6):671-4. DOI: 10.1016/j.cell.2004.09.005. View

14.

Hayat M, Howlader N, Reichman M, Edwards B . Cancer statistics, trends, and multiple primary cancer analyses from the Surveillance, Epidemiology, and End Results (SEER) Program. Oncologist. 2007; 12(1):20-37. DOI: 10.1634/theoncologist.12-1-20. View

15.

Xie F, Liu J, Li C, Zhao Y . Simvastatin blocks TGF-β1-induced epithelial-mesenchymal transition in human prostate cancer cells. Oncol Lett. 2016; 11(5):3377-3383. PMC: 4840973. DOI: 10.3892/ol.2016.4404. View

16.

De Marzo A, DeWeese T, Platz E, Meeker A, Nakayama M, Epstein J . Pathological and molecular mechanisms of prostate carcinogenesis: implications for diagnosis, detection, prevention, and treatment. J Cell Biochem. 2004; 91(3):459-77. DOI: 10.1002/jcb.10747. View

17.

Mitselou A, Galani V, Skoufi U, Arvanitis D, Lampri E, Ioachim E . Syndecan-1, Epithelial-Mesenchymal Transition Markers (E-cadherin/β-catenin) and Neoangiogenesis-related Proteins (PCAM-1 and Endoglin) in Colorectal Cancer. Anticancer Res. 2016; 36(5):2271-80. View

18.

De Marzo A, Marchi V, Epstein J, Nelson W . Proliferative inflammatory atrophy of the prostate: implications for prostatic carcinogenesis. Am J Pathol. 1999; 155(6):1985-92. PMC: 1866955. DOI: 10.1016/S0002-9440(10)65517-4. View

19.

Riemann A, Schneider B, Ihling A, Nowak M, Sauvant C, Thews O . Acidic environment leads to ROS-induced MAPK signaling in cancer cells. PLoS One. 2011; 6(7):e22445. PMC: 3144229. DOI: 10.1371/journal.pone.0022445. View

20.

Vyas H, Krishnaswamy G . Paul Ehrlich's "Mastzellen"--from aniline dyes to DNA chip arrays: a historical review of developments in mast cell research. Methods Mol Biol. 2005; 315:3-11. View