Spliceosome Profiling Visualizes Operations of a Dynamic RNP at Nucleotide Resolution

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2018 May 5

PMID 29727661

Citations 20

Authors

Jordan E Burke

Adam D Longhurst

Daria Merkurjev

Jade Sales-Lee

Beiduo Rao

James J Moresco

John R Yates 3rd

Jingyi Jessica Li

Hiten D Madhani

Affiliations

Soon will be listed here.

Abstract

Tools to understand how the spliceosome functions in vivo have lagged behind advances in the structural biology of the spliceosome. Here, methods are described to globally profile spliceosome-bound pre-mRNA, intermediates, and spliced mRNA at nucleotide resolution. These tools are applied to three yeast species that span 600 million years of evolution. The sensitivity of the approach enables the detection of canonical and non-canonical events, including interrupted, recursive, and nested splicing. This application of statistical modeling uncovers independent roles for the size and position of the intron and the number of introns per transcript in substrate progression through the two catalytic stages. These include species-specific inputs suggestive of spliceosome-transcriptome coevolution. Further investigations reveal the ATP-dependent discard of numerous endogenous substrates after spliceosome assembly in vivo and connect this discard to intron retention, a form of splicing regulation. Spliceosome profiling is a quantitative, generalizable global technology used to investigate an RNP central to eukaryotic gene expression.

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