Elective Single Embryo Transfer: Comparison of Blastocyst and Cleavage-stage Embryo Transfer

Overview

Journal Reprod Med Biol

Specialty Biology

Date 2018 Apr 28

PMID 29699223

Citations 1

Authors

Toshihiro Kawamura

Michiko Mori

Akane Arichi

Yuko Tajima

Yasuhiro Karasawa

Kahori Suga

Sachiko Ikumi

Seika Ishikawa

Makiko Kawamura

Affiliations

Soon will be listed here.

Abstract

Blastocysts are reportedly suitable for preventing multiple pregnancies as a result of the high implantation rate per embryo. The present study compared clinical results for elective single embryo transfer (ET) between blastocysts and cleavage-stage embryos in order to ascertain the usefulness of blastocyst culturing in single ET. Between January 2002 and December 2004, conventional fertilization ET and/or intracytoplasmic sperm injection was carried out for single ET in 86 cycles, to prevent multiple pregnancies (for medical reasons or because of patient wishes). Among the 80 cycles in which a fresh embryo was transferred, pregnancy/implantation rates per ET were 35.3% for day 2/3 ET and 50.0% for day 5 ET, and pregnancy/implantation rates per oocyte retrieval were 35.3% for day 2/3 ET and 44.2% for day 5 ET. Ongoing pregnancy/delivery rates per oocyte retrieval were 32.4% for day 2/3 ET and 38.5% for day 5 ET. Monozygotic twinning occurred in one case of day 5 ET. Pregnancy rates per single ET tended to be higher for day 5 ET than for day 2/3 ET. However, no marked differences were identified in ongoing pregnancy/delivery rates per oocyte retrieval between groups. (Reprod Med Biol 2005; : 197-201).

Citing Articles

Effect on clinical and neonatal outcomes of blastocelic microsuction prior to vitrification.

Mitsuhata S, Endo Y, Hayashi M, Fujii Y, Motoyama H Reprod Med Biol. 2019; 18(3):284-289.

PMID: 31312108 PMC: 6613010. DOI: 10.1002/rmb2.12273.

References

Bavister B, Boatman D . The neglected human blastocyst revisited. Hum Reprod. 1997; 12(8):1607-10. DOI: 10.1093/oxfordjournals.humrep.a019596. View

Blake D, Proctor M, Johnson N . The merits of blastocyst versus cleavage stage embryo transfer: a Cochrane review. Hum Reprod. 2004; 19(4):795-807. DOI: 10.1093/humrep/deh104. View

Desai N . The road to blastocyst transfer. Hum Reprod. 1999; 13(12):3292-4. DOI: 10.1093/oxfordjournals.humrep.a019678. View

Utsunomiya T, Ito H, Nagaki M, Sato J . A prospective, randomized study: day 3 versus hatching blastocyst stage. Hum Reprod. 2004; 19(7):1598-603. DOI: 10.1093/humrep/deh288. View

Gardner D, Lane M . Blastocyst transfer. Clin Obstet Gynecol. 2003; 46(2):231-8. DOI: 10.1097/00003081-200306000-00005. View

Slotnick R, Ortega J . Monoamniotic twinning and zona manipulation: a survey of U.S. IVF centers correlating zona manipulation procedures and high-risk twinning frequency. J Assist Reprod Genet. 1996; 13(5):381-5. DOI: 10.1007/BF02066168. View

Jones G, Trounson A, Gardner D, Kausche A, Lolatgis N, Wood C . Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod. 1998; 13(1):169-77. DOI: 10.1093/humrep/13.1.169. View

Behr B, Fisch J, Racowsky C, Miller K, Pool T, Milki A . Blastocyst-ET and monozygotic twinning. J Assist Reprod Genet. 2000; 17(6):349-51. PMC: 3455394. DOI: 10.1023/a:1009461213139. View

Kuramoto T, Boediono A, Egashira A, Motoishi M, Sugioka M, Fukuda K . Selected single blastocyst transfers maintained pregnancy outcome and eliminated multiple pregnancies. Reprod Med Biol. 2018; 3(1):13-18. PMC: 5891773. DOI: 10.1111/j.1447-0578.2004.00046.x. View

10.

Duncombe G, Dickinson J, Evans S . Perinatal characteristics and outcomes of pregnancies complicated by twin-twin transfusion syndrome. Obstet Gynecol. 2003; 101(6):1190-6. DOI: 10.1016/s0029-7844(03)00231-x. View

11.

Emiliani S, Delbaere A, Vannin A, Biramane J, Verdoodt M, Englert Y . Similar delivery rates in a selected group of patients, for day 2 and day 5 embryos both cultured in sequential medium: a randomized study. Hum Reprod. 2003; 18(10):2145-50. DOI: 10.1093/humrep/deg394. View

12.

Milki A, Jun S, Hinckley M, Behr B, Giudice L, Westphal L . Incidence of monozygotic twinning with blastocyst transfer compared to cleavage-stage transfer. Fertil Steril. 2003; 79(3):503-6. DOI: 10.1016/s0015-0282(02)04754-4. View

13.

da Motta E, Alegretti J, Baracat E, Olive D, Serafini P . High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media. Fertil Steril. 1998; 70(4):659-63. DOI: 10.1016/s0015-0282(98)00263-5. View

14.

Wilson M, Hartke K, Kiehl M, Rodgers J, Brabec C, Lyles R . Integration of blastocyst transfer for all patients. Fertil Steril. 2002; 77(4):693-6. DOI: 10.1016/s0015-0282(01)03235-6. View

15.

Menezo Y, Hamamah S, Hazout A, Dale B . Time to switch from co-culture to sequential defined media for transfer at the blastocyst stage. Hum Reprod. 1998; 13(8):2043-4. DOI: 10.1093/humrep/13.8.2043. View

16.

Fanchin R, Ayoubi J, Righini C, Olivennes F, Schonauer L, Frydman R . Uterine contractility decreases at the time of blastocyst transfers. Hum Reprod. 2001; 16(6):1115-9. DOI: 10.1093/humrep/16.6.1115. View

17.

Seng Y, Rajadurai V . Twin-twin transfusion syndrome: a five year review. Arch Dis Child Fetal Neonatal Ed. 2000; 83(3):F168-70. PMC: 1721171. DOI: 10.1136/fn.83.3.f168. View

18.

Gardner D, Lane M . Culture and selection of viable blastocysts: a feasible proposition for human IVF?. Hum Reprod Update. 1997; 3(4):367-82. DOI: 10.1093/humupd/3.4.367. View

19.

Wenstrom K, Syrop C, Hammitt D, Van Voorhis B . Increased risk of monochorionic twinning associated with assisted reproduction. Fertil Steril. 1993; 60(3):510-4. DOI: 10.1016/s0015-0282(16)56169-x. View

20.

Alikani M, Cekleniak N, Walters E, Cohen J . Monozygotic twinning following assisted conception: an analysis of 81 consecutive cases. Hum Reprod. 2003; 18(9):1937-43. DOI: 10.1093/humrep/deg369. View