Comparative Analysis of Cadmium-induced Stress Responses by the Aromatic and Non-aromatic Rice Genotypes of West Bengal

Overview

Journal Environ Sci Pollut Res Int

Publisher Springer

Specialties Environmental Health
Toxicology

Date 2018 Apr 27

PMID 29696542

Citations 1

Authors

Snehalata Majumdar

Bratati Chakraborty

Rita Kundu

Affiliations

Soon will be listed here.

Abstract

Constant exposure of the living ecosystems to heavy metals, like cadmium (Cd), induces a detectable change at the biochemical and genetic level. Repeated application of phosphate fertilizers in paddy fields, leads to increase in Cd content of soil. Cd being highly mobile is transported to shoot and grain, thereby entering into the food chain of animal system. In the present study, treatment of 7-day old rice seedlings with 10 μM cadmium chloride resulted in Cd toxicity across the seven non-aromatic and six aromatic rice cultivars and landraces used for the study. Free proline and malondialdehyde content of treated samples were higher in comparison to the untreated samples, which indicated Cd induced tissue damage in plants. Photosynthetic pigment content of treated samples was also found to be much lower in comparison to the untreated samples, which is probably due to peroxidation of membrane, leading to compromised and lower photosynthetic efficiency of treated plants. At the genetic level, Randomly Amplified Polymorphic DNA assay was found to efficiently detect the genetic polymorphisms (caused by alterations in DNA bases) induced by Cd. Production of unique polymorphic bands in Cd-treated plants helps in assessment of the degree of damage Cd imparts on the plant system. Cluster analysis was performed and the rice genotypes were grouped into five distinct clusters, with IR64 and Tulsibhog in two distinct groups. Based on the variability in responses, the 13 rice genotypes were grouped into sensitive and tolerant ones.

Citing Articles

Morpho-Physiological Adaptations of Rice Cultivars Under Heavy Metal Stress: A Systematic Review and Meta-Analysis.

Espinola E, Cabreros M, Redillas M Life (Basel). 2025; 15(2).

PMID: 40003598 PMC: 11856324. DOI: 10.3390/life15020189.

Physiological and biochemical mechanisms of grain yield loss in fumitory (Fumaria parviflora Lam.) exposed to copper and drought stress.

Tashakorizadeh M, Golkar P, Vahabi M, Ghorbanpour M Sci Rep. 2023; 13(1):17934.

PMID: 37863928 PMC: 10589251. DOI: 10.1038/s41598-023-45103-5.

References

Cenkci S, Yildiz M, Cigerci I, Konuk M, Bozdag A . Toxic chemicals-induced genotoxicity detected by random amplified polymorphic DNA (RAPD) in bean (Phaseolus vulgaris L.) seedlings. Chemosphere. 2009; 76(7):900-6. DOI: 10.1016/j.chemosphere.2009.05.001. View

Srivastava R, Pandey P, Rajpoot R, Rani A, Dubey R . Cadmium and lead interactive effects on oxidative stress and antioxidative responses in rice seedlings. Protoplasma. 2014; 251(5):1047-65. DOI: 10.1007/s00709-014-0614-3. View

Arnon D . COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949; 24(1):1-15. PMC: 437905. DOI: 10.1104/pp.24.1.1. View

Yu Z, Zhou Q . Growth responses and cadmium accumulation of Mirabilis jalapa L. under interaction between cadmium and phosphorus. J Hazard Mater. 2009; 167(1-3):38-43. DOI: 10.1016/j.jhazmat.2008.12.082. View

Daud M, Sun Y, Dawood M, Hayat Y, Variath M, Wu Y . Cadmium-induced functional and ultrastructural alterations in roots of two transgenic cotton cultivars. J Hazard Mater. 2008; 161(1):463-73. DOI: 10.1016/j.jhazmat.2008.03.128. View

Atienzar , Cordi , Donkin , Evenden , Jha , Depledge . Comparison of ultraviolet-induced genotoxicity detected by random amplified polymorphic DNA with chlorophyll fluorescence and growth in a marine macroalgae, Palmaria palmata. Aquat Toxicol. 2000; 50(1-2):1-12. DOI: 10.1016/s0166-445x(99)00100-9. View

Atienzar F, Conradi M, Evenden A, Jha A, Depledge M . Qualitative assessment of genotoxicity using random amplified polymorphic DNA: Comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene. Environ Toxicol Chem. 2018; 18(10):2275-2282. DOI: 10.1002/etc.5620181023. View

Irfan M, Hayat S, Ahmad A, Alyemeni M . Soil cadmium enrichment: Allocation and plant physiological manifestations. Saudi J Biol Sci. 2013; 20(1):1-10. PMC: 3730847. DOI: 10.1016/j.sjbs.2012.11.004. View

Gupta M, Sarin N . Heavy metal induced DNA changes in aquatic macrophytes: Random amplified polymorphic DNA analysis and identification of sequence characterized amplified region marker. J Environ Sci (China). 2010; 21(5):686-90. DOI: 10.1016/s1001-0742(08)62324-4. View

10.

Srivastava S, Mishra S, Tripathi R, Dwivedi S, Trivedi P, Tandon P . Phytochelatins and antioxidant systems respond differentially during arsenite and arsenate stress in Hydrilla verticillata (L.f.) Royle. Environ Sci Technol. 2007; 41(8):2930-6. DOI: 10.1021/es062167j. View

11.

Cao D, Zhang H, Wang Y, Zheng L . Accumulation and distribution characteristics of zinc and cadmium in the hyperaccumulator plant Sedum plumbizincicola. Bull Environ Contam Toxicol. 2014; 93(2):171-6. DOI: 10.1007/s00128-014-1284-8. View

12.

Korpe D, Aras S . Evaluation of copper-induced stress on eggplant (Solanum melongena L.) seedlings at the molecular and population levels by use of various biomarkers. Mutat Res. 2010; 719(1-2):29-34. DOI: 10.1016/j.mrgentox.2010.10.003. View

13.

Heath R, Packer L . Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968; 125(1):189-98. DOI: 10.1016/0003-9861(68)90654-1. View

14.

Kosolsaksakul P, Farmer J, Oliver I, Graham M . Geochemical associations and availability of cadmium (Cd) in a paddy field system, northwestern Thailand. Environ Pollut. 2014; 187:153-61. DOI: 10.1016/j.envpol.2014.01.006. View

15.

Zhang F, Zhang H, Wang G, Xu L, Shen Z . Cadmium-induced accumulation of hydrogen peroxide in the leaf apoplast of Phaseolus aureus and Vicia sativa and the roles of different antioxidant enzymes. J Hazard Mater. 2009; 168(1):76-84. DOI: 10.1016/j.jhazmat.2009.02.002. View

16.

Singh S, Eapen S, DSouza S . Cadmium accumulation and its influence on lipid peroxidation and antioxidative system in an aquatic plant, Bacopa monnieri L. Chemosphere. 2005; 62(2):233-46. DOI: 10.1016/j.chemosphere.2005.05.017. View

17.

Liu W, Li P, Qi X, Zhou Q, Zheng L, Sun T . DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis. Chemosphere. 2005; 61(2):158-67. DOI: 10.1016/j.chemosphere.2005.02.078. View

18.

Uraguchi S, Mori S, Kuramata M, Kawasaki A, Arao T, Ishikawa S . Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice. J Exp Bot. 2009; 60(9):2677-88. PMC: 2692013. DOI: 10.1093/jxb/erp119. View

19.

Labra M, Di Fabio T, Grassi F, Regondi S, Bracale M, Vannini C . AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants. Chemosphere. 2003; 52(7):1183-8. DOI: 10.1016/S0045-6535(03)00367-9. View

20.

Moradi A, Abbaspour K, Afyuni M . Modelling field-scale cadmium transport below the root zone of a sewage sludge amended soil in an arid region in Central Iran. J Contam Hydrol. 2005; 79(3-4):187-206. DOI: 10.1016/j.jconhyd.2005.07.005. View