Identification of Key Candidate Genes and Pathways in Endometrial Cancer by Integrated Bioinformatical Analysis

Overview

Journal Asian Pac J Cancer Prev

Specialty Oncology

Date 2018 Apr 26

PMID 29693365

Citations 15

Authors

Lihong Liu

Fangxu Chen

Aihui Xiu

Bo Du

Hao Ai

Wei Xie

Affiliations

Soon will be listed here.

Abstract

Endometrial Cancer is the most common female genital tract malignancy, its pathogenesis is complex, not yet fully described. To identify key genes of Endometrial Cancer we downloaded the gene chip GSE17025 from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were identified through the GEO2R analysis tool. Functional and pathway enrichment analysis were performed for DEGs using DAVID database. The network of protein–protein-interaction (PPI) was established by STRING website and visualized by Cytoscape. Then, functional and pathway enrichment analysis of DEGS were performed by DAVID database. A total of 1000 significant differences genes were obtained, contain 362 up-regulated genes and 638 down-regulated genes. PCDH10, SLC6A2, OGN, SFRP4, TRH, ANGPTL, FOSB are down-regulated genes. The gene of IGH, CCL20, ELF5, LTF, ASPM expression level in tumor patients are up-regulated. Biological function of enrichment include metabolism of xenobiotics by cytochrome P450, MAPK signaling pathway, Serotonergic synapse, Protein digestion and absorption, IL-17 signaling pathway, Chemokine signaling pathway, HIF-1 signaling pathway, p53 signaling pathway. All in all, the current study to determine endometrial differentially expressed genes and biological function, comprehensive analysis of intrauterine membrane carcinoma pathogenesis mechanism, and might be used as molecular targets and diagnostic biomarkers for the treatment of endometrial cancer.

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References

Bersinger N, Schneider B, Vorburger S, Johann S, Candinas D, Mueller M . Prognostic value of tumour endothelial markers in patients with endometrial cancer. Oncol Lett. 2012; 1(1):203-207. PMC: 3436417. DOI: 10.3892/ol_00000037. View

Zhao Y, Yang Y, Trovik J, Sun K, Zhou L, Jiang P . A novel wnt regulatory axis in endometrioid endometrial cancer. Cancer Res. 2014; 74(18):5103-17. DOI: 10.1158/0008-5472.CAN-14-0427. View

Husseinzadeh N, Davenport S . Role of toll-like receptors in cervical, endometrial and ovarian cancers: a review. Gynecol Oncol. 2014; 135(2):359-63. DOI: 10.1016/j.ygyno.2014.08.013. View

Barrett T, Wilhite S, Ledoux P, Evangelista C, Kim I, Tomashevsky M . NCBI GEO: archive for functional genomics data sets--update. Nucleic Acids Res. 2012; 41(Database issue):D991-5. PMC: 3531084. DOI: 10.1093/nar/gks1193. View

Piulats J, Guerra E, Gil-Martin M, Roman-Canal B, Gatius S, Sanz-Pamplona R . Molecular approaches for classifying endometrial carcinoma. Gynecol Oncol. 2017; 145(1):200-207. DOI: 10.1016/j.ygyno.2016.12.015. View

Chen W, Zheng R, Baade P, Zhang S, Zeng H, Bray F . Cancer statistics in China, 2015. CA Cancer J Clin. 2016; 66(2):115-32. DOI: 10.3322/caac.21338. View

Wu L, Chen X, Zhao J, Martin B, Zepp J, Ko J . A novel IL-17 signaling pathway controlling keratinocyte proliferation and tumorigenesis via the TRAF4-ERK5 axis. J Exp Med. 2015; 212(10):1571-87. PMC: 4577838. DOI: 10.1084/jem.20150204. View

Siegel R, Miller K, Jemal A . Cancer statistics, 2016. CA Cancer J Clin. 2016; 66(1):7-30. DOI: 10.3322/caac.21332. View

Xu Z, Zhou Y, Cao Y, Dinh T, Wan J, Zhao M . Identification of candidate biomarkers and analysis of prognostic values in ovarian cancer by integrated bioinformatics analysis. Med Oncol. 2016; 33(11):130. DOI: 10.1007/s12032-016-0840-y. View

10.

Chuwa A, Sone K, Oda K, Ikeda Y, Fukuda T, Wada-Hiraike O . Significance of survivin as a prognostic factor and a therapeutic target in endometrial cancer. Gynecol Oncol. 2016; 141(3):564-569. DOI: 10.1016/j.ygyno.2016.04.003. View

11.

Kandoth C, Schultz N, Cherniack A, Akbani R, Liu Y, Shen H . Integrated genomic characterization of endometrial carcinoma. Nature. 2013; 497(7447):67-73. PMC: 3704730. DOI: 10.1038/nature12113. View

12.

Huang D, Sherman B, Lempicki R . Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009; 4(1):44-57. DOI: 10.1038/nprot.2008.211. View

13.

Di Domenico M, Santoro A, Ricciardi C, Iaccarino M, Iaccarino S, Freda M . Epigenetic fingerprint in endometrial carcinogenesis: the hypothesis of a uterine field cancerization. Cancer Biol Ther. 2011; 12(5):447-57. DOI: 10.4161/cbt.12.5.15963. View

14.

Ye M, Li J, Gong J . PCDH10 gene inhibits cell proliferation and induces cell apoptosis by inhibiting the PI3K/Akt signaling pathway in hepatocellular carcinoma cells. Oncol Rep. 2017; 37(6):3167-3174. PMC: 5442397. DOI: 10.3892/or.2017.5630. View

15.

Bregar A, Deshpande A, Grange C, Zi T, Stall J, Hirsch H . Characterization of immune regulatory molecules B7-H4 and PD-L1 in low and high grade endometrial tumors. Gynecol Oncol. 2017; 145(3):446-452. DOI: 10.1016/j.ygyno.2017.03.006. View

16.

Gao H, Wang H, Yang W . Identification of key genes and construction of microRNA-mRNA regulatory networks in multiple myeloma by integrated multiple GEO datasets using bioinformatics analysis. Int J Hematol. 2017; 106(1):99-107. DOI: 10.1007/s12185-017-2216-2. View

17.

Gayyed M, El-Maqsoud N, Tawfiek E, El Gelany S, Rahman M . A comprehensive analysis of CDC20 overexpression in common malignant tumors from multiple organs: its correlation with tumor grade and stage. Tumour Biol. 2015; 37(1):749-62. DOI: 10.1007/s13277-015-3808-1. View

18.

Zhong X, Shen H, Mao J, Zhang J, Han W . Epigenetic silencing of protocadherin 10 in colorectal cancer. Oncol Lett. 2017; 13(4):2449-2453. PMC: 5403191. DOI: 10.3892/ol.2017.5733. View

19.

Xue L, Xu Z, Wang K, Wang N, Zhang X, Wang S . Regulatory network of microRNAs, target genes, transcription factors and host genes in endometrial cancer. Asian Pac J Cancer Prev. 2015; 16(2):475-83. DOI: 10.7314/apjcp.2015.16.2.475. View

20.

Barrett T, Troup D, Wilhite S, Ledoux P, Rudnev D, Evangelista C . NCBI GEO: archive for high-throughput functional genomic data. Nucleic Acids Res. 2008; 37(Database issue):D885-90. PMC: 2686538. DOI: 10.1093/nar/gkn764. View