Hemolytic, Anticancer and Antigiardial Activity of Venom

Overview

Journal J Venom Anim Toxins Incl Trop Dis

Date 2018 Apr 26

PMID 29692802

Citations 8

Authors

Fernando Lazcano-Perez

Ariana Zavala-Moreno

Yadira Rufino-Gonzalez

Martha Ponce-Macotela

Alejandro Garcia-Arredondo

Miguel Cuevas-Cruz

Saul Gomez-Manzo

Jaime Marcial-Quino

Barbarin Arreguin-Lozano

Roberto Arreguin-Espinosa

Affiliations

Soon will be listed here.

Abstract

Background: Cnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite .

Methods: The presence of cytolysins and vasoconstrictor activity of venom were determined by hemolysis, PLA and isolated rat aortic ring assays, respectively. The cytotoxic effect was tested on HCT-15 (human colorectal adenocarcinoma), MCF-7 (human mammary adenocarcinoma), K562 (human chronic myelogenous leukemia), U251 (human glyoblastoma), PC-3 (human prostatic adenocarcinoma) and SKLU-1 (human lung adenocarcinoma). An in vivo toxicity assay was performed with crickets and the antiparasitic assay was performed against at 24 h of incubation.

Results: venom produced hemolytic and PLA activity and showed specific cytotoxicity against U251 and SKLU-1 cell lines, with approximately 50% growing inhibition. The venom was toxic to insects and showed activity against in a dose-dependent manner by possibly altering its membrane osmotic equilibrium.

Conclusion: These results suggest that venom contains compounds with potential therapeutic value against microorganisms and cancer.

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