Production of Isotope-labeled Proteins in Insect Cells for NMR

Overview

Journal J Biomol NMR

Publisher Springer

Specialties Molecular Biology
Nuclear Medicine

Date 2018 Apr 25

PMID 29687312

Citations 26

Authors

Bastian Franke

Christian Opitz

Shin Isogai

Anne Grahl

Leonildo Delgado

Alvar D Gossert

Stephan Grzesiek

Affiliations

Soon will be listed here.

Abstract

Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.

Citing Articles

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