Metabolomic Profiling of Human Spermatozoa in Idiopathic Asthenozoospermia Patients Using Gas Chromatography-Mass Spectrometry

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2018 Apr 24

PMID 29682560

Citations 27

Authors

Kai Zhao

Jianzhong Zhang

Zhen Xu

Yue Xu

Aiming Xu

Wei Chen

Chenkui Miao

Shouyong Liu

Zengjun Wang

Ruipeng Jia

Affiliations

Soon will be listed here.

Abstract

The purpose of this study was to describe the first metabolic profile of human sperm cells through the application of an untargeted platform based on gas chromatography-mass spectrometry (GC-MS). Sperm cell samples from patients diagnosed with idiopathic asthenozoospermia ( = 30) and healthy subjects ( = 30) were analyzed using a nontargeted metabolomics method based on GC-MS spectroscopy. The mass spectrometric data were collected using multivariate and univariate analyses to identify metabolites related to idiopathic asthenozoospermia. By using metabolomic strategies, we identified 33 metabolites, 27 of which were decreased in the idiopathic asthenozoospermia group compared with the normozoospermic group and six were increased in idiopathic asthenozoospermia. With respect to human sperm cells, some of these metabolites are reported here for the first time. Pathways for nucleoside, amino acid and energy metabolism, and the Krebs cycle were disturbed and were associated with idiopathic asthenozoospermia. The metabolic profiling provides an important first step in studying the pathophysiological mechanisms involved in IAS, and the identified metabolites may become potential biomarkers for its diagnosis and treatment.

Citing Articles

Elevated Lipid Concentrations in Seminal Plasma Can Reduce Sperm Motility in Simmental Bulls.

Yang Z, Luo F, Song C, Ma Z, Tian Y, Fu Y Animals (Basel). 2025; 15(2).

PMID: 39858276 PMC: 11762868. DOI: 10.3390/ani15020276.

Metabolomic signature of sperm in men with obesity-associated asthenozoospermia.

Hou Y, Wang J, Pan M, Zhou Y, Wang Y, Chen J J Assist Reprod Genet. 2024; 41(11):3161-3171.

PMID: 39432192 PMC: 11621289. DOI: 10.1007/s10815-024-03294-4.

Quantitative Analysis of the Human Semen Phosphorometabolome by P-NMR.

Serrano R, Martin-Hidalgo D, Bilbao J, Bernardo-Seisdedos G, Millet O, Garcia-Marin L Int J Mol Sci. 2024; 25(3).

PMID: 38338962 PMC: 10855173. DOI: 10.3390/ijms25031682.

The Role of Chorein Deficiency in Late Spermatogenesis.

Arai K, Nishizawa Y, Nagata O, Sakimoto H, Sasaki N, Sano A Biomedicines. 2024; 12(1).

PMID: 38275411 PMC: 10813020. DOI: 10.3390/biomedicines12010240.

The Role of Circular RNAs in Male Infertility and Reproductive Cancers: A Narrative Review.

Derakhshan Z, Bahmanpour S, Alaee S, Fallahi J, Tabei S Iran J Med Sci. 2023; 48(6):527-541.

PMID: 38094281 PMC: 10715113. DOI: 10.30476/IJMS.2022.95302.2661.

References

Ortega C, Verheyen G, Raick D, Camus M, Devroey P, Tournaye H . Absolute asthenozoospermia and ICSI: what are the options?. Hum Reprod Update. 2011; 17(5):684-92. DOI: 10.1093/humupd/dmr018. View

Gilany K, Moazeni-Pourasil R, Jafarzadeh N, Savadi-Shiraz E . Metabolomics fingerprinting of the human seminal plasma of asthenozoospermic patients. Mol Reprod Dev. 2013; 81(1):84-6. DOI: 10.1002/mrd.22284. View

Jelks K, Berger T, Horner C, Miller M . alpha-chlorohydrin induced changes in sperm fertilizing ability in the rat: association with diminished sperm ATP levels and motility. Reprod Toxicol. 2001; 15(1):11-20. DOI: 10.1016/s0890-6238(00)00115-5. View

Zhang S, Wang Q, Ding X, Wang T, Mu X, Chen Z . Association of polymorphisms in PATE1 gene with idiopathic asthenozoospermia in Sichuan, China. J Reprod Immunol. 2016; 118:54-60. DOI: 10.1016/j.jri.2016.08.007. View

Kovac J, Pastuszak A, Lamb D . The use of genomics, proteomics, and metabolomics in identifying biomarkers of male infertility. Fertil Steril. 2013; 99(4):998-1007. PMC: 3652543. DOI: 10.1016/j.fertnstert.2013.01.111. View

Newsholme P, Lima M, Procopio J, Pithon-Curi T, Doi S, Bazotte R . Glutamine and glutamate as vital metabolites. Braz J Med Biol Res. 2003; 36(2):153-63. DOI: 10.1590/s0100-879x2003000200002. View

Cheng Y, Yang X, Deng X, Zhang X, Li P, Tao J . Metabolomics in bladder cancer: a systematic review. Int J Clin Exp Med. 2015; 8(7):11052-63. PMC: 4565288. View

Young V . Adult amino acid requirements: the case for a major revision in current recommendations. J Nutr. 1994; 124(8 Suppl):1517S-1523S. DOI: 10.1093/jn/124.suppl_8.1517S. View

Postic C, Dentin R, Girard J . Role of the liver in the control of carbohydrate and lipid homeostasis. Diabetes Metab. 2005; 30(5):398-408. DOI: 10.1016/s1262-3636(07)70133-7. View

10.

Kim S, Kim S, Kim J, Hwang S, Yoo H . Understanding Metabolomics in Biomedical Research. Endocrinol Metab (Seoul). 2015; 31(1):7-16. PMC: 4803564. DOI: 10.3803/EnM.2016.31.1.7. View

11.

Esteves S . Novel concepts in male factor infertility: clinical and laboratory perspectives. J Assist Reprod Genet. 2016; 33(10):1319-1335. PMC: 5065546. DOI: 10.1007/s10815-016-0763-8. View

12.

Ducker G, Rabinowitz J . One-Carbon Metabolism in Health and Disease. Cell Metab. 2016; 25(1):27-42. PMC: 5353360. DOI: 10.1016/j.cmet.2016.08.009. View

13.

Guo J, Zhao Y, Huang W, Hu W, Gu J, Chen C . Sperm motility inversely correlates with seminal leptin levels in idiopathic asthenozoospermia. Int J Clin Exp Med. 2014; 7(10):3550-5. PMC: 4238556. View

14.

Shen S, Wang J, Liang J, He D . Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia. World J Urol. 2013; 31(6):1395-401. DOI: 10.1007/s00345-013-1023-5. View

15.

Gogol P, Szczesniak-Fabianczyk B, Wierzchos-Hilczer A . The photon emission, ATP level and motility of boar spermatozoa during liquid storage. Reprod Biol. 2009; 9(1):39-49. DOI: 10.1016/s1642-431x(12)60093-x. View

16.

Letter A, Zombor G, HENDERSON J . Tryptophan as a source of one-carbon units for purine biosynthesis de novo. Can J Biochem. 1973; 51(4):486-8. DOI: 10.1139/o73-058. View

17.

Paiva C, Amaral A, Rodriguez M, Canyellas N, Correig X, Ballesca J . Identification of endogenous metabolites in human sperm cells using proton nuclear magnetic resonance ((1) H-NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Andrology. 2015; 3(3):496-505. DOI: 10.1111/andr.12027. View

18.

Rivera Egea R, Garrido Puchalt N, Meseguer Escriva M, Varghese A . OMICS: Current and future perspectives in reproductive medicine and technology. J Hum Reprod Sci. 2014; 7(2):73-92. PMC: 4150148. DOI: 10.4103/0974-1208.138857. View

19.

Tomoike F, Nakagawa N, Kuramitsu S, Masui R . Structural and Biochemical Studies on the Reaction Mechanism of Uridine-Cytidine Kinase. Protein J. 2015; 34(6):411-20. DOI: 10.1007/s10930-015-9636-8. View

20.

Lill R, Muhlenhoff U . Iron-sulfur protein biogenesis in eukaryotes: components and mechanisms. Annu Rev Cell Dev Biol. 2006; 22:457-86. DOI: 10.1146/annurev.cellbio.22.010305.104538. View