Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2018 Apr 24

PMID 29682543

Citations 5

Authors

Xiangju Wu

Jing Qi

Xiaoyan Cong

Lei Chen

Yue Hu

Dongwan Yoo

Guisheng Wang

Fulin Tian

Feng Li

Wenbo Sun

Zhi Chen

Lihui Guo

Jiaqiang Wu

Jun Li

Jinbao Wang

Xiaomin Zhao

Yijun Du

Affiliations

Soon will be listed here.

Abstract

Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.

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