Is There a Correlation Between and Enterohepatic Helicobacter Species and Gallstone Cholecystitis?

Overview

Journal Middle East J Dig Dis

Specialty Gastroenterology

Date 2018 Apr 24

PMID 29682244

Citations 7

Authors

Seyed Masih Fatemi

Abbas Doosti

Dariush Shokri

Sadegh Ghorbani-Dalini

Morteza Molazadeh

Hossein Tavakoli

Mohammad Minakari

Hamid Tavakkoli

Affiliations

Soon will be listed here.

Abstract

BACKGROUND Cholecystitis is a common surgical condition. Recently, several authors have reported that DNA of bile tolerant spp. has been found in the human bile colonizing the biliary tract. The aim of this study was to evaluate the association between the presence of spp. and gallstone cholecystitis. METHODS In this case-control study, gallstones, bile, and gallbladder mucosa were collected from 25 patients without gallstone disease, 24 with acute cholecystitis, and 28 with chronic cholecystitis. The presence of , and were investigated by polymerase chain reaction (PCR) using species-specific primers. RESULTS In this study, 77 subjects with acute and chronic cholecystitis and control groups with a mean age of 46.85 ± 14.53 years, including 58 (67.25%) women and 19 (32.75%) men were included. DNA of 10 spp. was detected in the bile of the patients with cholecystitis including eight and two . However, we could not detect and DNA in the samples. Moreover, there was an association between and acute cholecystitis ( = 0.048), which was found to be stronger in 31-40-year-olds group ( = 0.003). CONCLUSION We found an association between the presence of DNA and acute gallstone cholecystitis. There is not statistically significant correlation between three enterohepatic spp. ( , and ) and cholelithiasis. Given the low sample size of the patients, more studies are required to clear the clinical role of spp. in the gallstone disease and cholecystitis.

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References

Stanley J, Linton D, Burnens A, Dewhirst F, On S, Porter A . Helicobacter pullorum sp. nov.-genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology (Reading). 1994; 140 ( Pt 12):3441-9. DOI: 10.1099/13500872-140-12-3441. View

Suzuki H, Iwasaki E, Hibi T . Helicobacter pylori and gastric cancer. Gastric Cancer. 2009; 12(2):79-87. DOI: 10.1007/s10120-009-0507-x. View

Richards C, Buchholz B, Ford T, Broadaway S, Pyle B, Camper A . Optimizing the growth of stressed Helicobacter pylori. J Microbiol Methods. 2010; 84(2):174-82. PMC: 3034813. DOI: 10.1016/j.mimet.2010.11.015. View

Monteiro L, Gras N, Vidal R, Cabrita J, Megraud F . Detection of Helicobacter pylori DNA in human feces by PCR: DNA stability and removal of inhibitors. J Microbiol Methods. 2001; 45(2):89-94. DOI: 10.1016/s0167-7012(01)00225-1. View

Khedmat H, Karbasi-Afshar R, Agah S, Taheri S . Helicobacter pylori Infection in the general population: A Middle Eastern perspective. Caspian J Intern Med. 2013; 4(4):745-53. PMC: 3841773. View

Apostolov E, Abu Al-Soud W, Nilsson I, Kornilovska I, Usenko V, Lyzogubov V . Helicobacter pylori and other Helicobacter species in gallbladder and liver of patients with chronic cholecystitis detected by immunological and molecular methods. Scand J Gastroenterol. 2005; 40(1):96-102. DOI: 10.1080/00365520410009546. View

Singh V, Mishra S, Rao G, Jain A, Dixit V, Gulati A . Evaluation of nested PCR in detection of Helicobacter pylori targeting a highly conserved gene: HSP60. Helicobacter. 2008; 13(1):30-4. PMC: 2752369. DOI: 10.1111/j.1523-5378.2008.00573.x. View

Maurer K, Rogers A, Ge Z, Wiese A, Carey M, Fox J . Helicobacter pylori and cholesterol gallstone formation in C57L/J mice: a prospective study. Am J Physiol Gastrointest Liver Physiol. 2005; 290(1):G175-82. DOI: 10.1152/ajpgi.00272.2005. View

Bulajic M, Maisonneuve P, Schneider-Brachert W, Muller P, Reischl U, Stimec B . Helicobacter pylori and the risk of benign and malignant biliary tract disease. Cancer. 2002; 95(9):1946-53. DOI: 10.1002/cncr.10893. View

10.

Kountouras J, Tsiaousi E, Trigonis S, Zavos C, Kouklakis G . Helicobacter pylori infection in a Greek cohort with biliary disease. Br J Biomed Sci. 2015; 71(4):178-9. DOI: 10.1080/09674845.2014.11669984. View

11.

Swidsinski A, Ludwig W, Pahlig H, Priem F . Molecular genetic evidence of bacterial colonization of cholesterol gallstones. Gastroenterology. 1995; 108(3):860-4. DOI: 10.1016/0016-5085(95)90461-1. View

12.

Forman D . Helicobacter pylori and gastric cancer. Scand J Gastroenterol Suppl. 1996; 220:23-6. View

13.

Griniatsos J, Sougioultzis S, Giaslakiotis K, Gazouli M, Prassas E, Felekouras E . Does Helicobacter pylori identification in the mucosa of the gallbladder correlate with cholesterol gallstone formation?. West Indian Med J. 2010; 58(5):428-32. View

14.

Avenaud P, Marais A, Monteiro L, Le Bail B, Bioulac Sage P, Balabaud C . Detection of Helicobacter species in the liver of patients with and without primary liver carcinoma. Cancer. 2000; 89(7):1431-9. View

15.

Neri V, Margiotta M, De Francesco V, Ambrosi A, Della Valle N, Fersini A . DNA sequences and proteic antigens of H. pylori in cholecystic bile and tissue of patients with gallstones. Aliment Pharmacol Ther. 2005; 22(8):715-20. DOI: 10.1111/j.1365-2036.2005.02644.x. View

16.

Shengelia M, Intskirveli N, Gogebashvili N . Inflammatory markers of gallstones disease in menopausal women. Georgian Med News. 2012; (208-209):52-5. View

17.

Fox J, Dewhirst F, Shen Z, Feng Y, Taylor N, Paster B . Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology. 1998; 114(4):755-63. DOI: 10.1016/s0016-5085(98)70589-x. View

18.

Blaser M, Chyou P, Nomura A . Age at establishment of Helicobacter pylori infection and gastric carcinoma, gastric ulcer, and duodenal ulcer risk. Cancer Res. 1995; 55(3):562-5. View

19.

Maurer K, Ihrig M, Rogers A, Ng V, Bouchard G, Leonard M . Identification of cholelithogenic enterohepatic helicobacter species and their role in murine cholesterol gallstone formation. Gastroenterology. 2005; 128(4):1023-33. DOI: 10.1053/j.gastro.2005.01.008. View

20.

Chen W, Li D, Cannan R, Stubbs R . Common presence of Helicobacter DNA in the gallbladder of patients with gallstone diseases and controls. Dig Liver Dis. 2003; 35(4):237-43. DOI: 10.1016/s1590-8658(03)00060-4. View