Cloning, Characterization, and Expression Analysis of Three Genes Encoding a Fatty Acid Desaturase from Seeds of

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2018 Apr 21

PMID 29673187

Citations 8

Authors

Jing Sun

Ming Chen

Mengyuan Zhu

Yu Jiang

Jiasong Meng

Daqiu Zhao

Jun Tao

Affiliations

Soon will be listed here.

Abstract

The gene catalyzes the conversion of diene fatty acids to triene fatty acids and is a key enzyme that determines the synthesis of alpha-linolenic acid. In this study, the full-length cDNAs of , , and are cloned from T. Hong & J. X. Zhang and named as , , and . Their open reading frame is 1203 bp, 1152 bp, and 1353 bp which encoded 400, 371, and 450 amino acids. The molecular weights of the amino acids are 46 kDa, 43 kDa, and 51 kDa while the isoelectric points are 7.34, 8.74, and 9.23, respectively. Bioinformatics analysis shows that all three genes are hydrophobic-hydrophobic, has three transmembrane domains, and and have two transmembrane domains. Multiple series alignment and phylogenetic analysis revealed that and are closely related while is more closely related to . Subcellular localization results showed that was located on the ER membrane and and were located on the chloroplast membrane. The relative expression level of in seeds is very high. expressed more in the ovary than the other two genes. was highly expressed in roots, stems, leaves, petals, and ovaries.

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