Effects of Upstream Shear Forces on Priming of Platelets for Downstream Adhesion and Activation

Overview

Journal Acta Biomater

Publisher Elsevier

Specialty Biomedical Engineering

Date 2018 Apr 15

PMID 29654993

Citations 15

Authors

Shekh M Rahman

Colin D Eichinger

Vladimir Hlady

Affiliations

Soon will be listed here.

Abstract

Statement Of Significance: A synthetic small-diameter vascular graft can often become stenotic due to intimal fibrous hyperplasia, either generally along the inside of the graft or at the anastomotic regions, leading to an increased shear force on flowing platelets. Our lab is studying how the upstream platelet preactivation (aka "priming") in flowing blood affects their downstream adhesion and activation. This manuscript describes a study in which priming of platelets is achieved by upstream stenotic narrowing in a microfluidic flow chamber. Such experimental design was intended to mimic a vascular implant with stenotic upstream anastomosis and downstream exposed platelet protein agonists. Understanding how the pre-activated platelets respond to imperfect vascular implant surfaces downstream is an important factor in designing better vascular implants.

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