Role of in Stress Tolerance and Adhesion Ability in KLDS1.0391

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2018 Apr 14

PMID 29651434

Citations 11

Authors

Fang-Fang Jia

Hui-Qi Zheng

Si-Rui Sun

Xue-Hui Pang

Yu Liang

Jia-Cui Shang

Zong-Tao Zhu

Xiang-Chen Meng

Affiliations

Soon will be listed here.

Abstract

, a probiotic, has a high survival rate and high colonization ability in the gastrointestinal tract. Tolerance to the gastrointestinal environment and adhesion to intestinal epithelial cells by some species (excluding ) are related to /AI-2. Here, the role of in tolerance to simulated digestive juice (SDJ) and adhesion to Caco-2 cells by KLDS1.0391 (hereafter, KLDS1.0391) was investigated. The KLDS1.0391 mutant strain was constructed by homologous recombination. When was deleted, acid and bile salt tolerance and survival rates in SDJ significantly decreased ( < 0.05 for all). The ability of the deletion strain to adhere to Caco-2 cells was markedly lower than that of the wild-type strain ( < 0.05). The ability of the mutant strain to adhere (competition, exclusion, and displacement) to ATCC 25922 was significantly lower than that of the wild-type strain ( < 0.05 for all). A significant decrease was noted only in the exclusion adhesion inhibition of the mutant strain to ATCC 14028 ( < 0.05). These results indicate that the gene plays an important role in the gastrointestinal environment tolerance and adhesion ability of KLDS1.0391.

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References

Xavier K, Bassler B . LuxS quorum sensing: more than just a numbers game. Curr Opin Microbiol. 2003; 6(2):191-7. DOI: 10.1016/s1369-5274(03)00028-6. View

Lebeer S, Claes I, Verhoeven T, Shen C, Lambrichts I, Ceuppens J . Impact of luxS and suppressor mutations on the gastrointestinal transit of Lactobacillus rhamnosus GG. Appl Environ Microbiol. 2008; 74(15):4711-8. PMC: 2519330. DOI: 10.1128/AEM.00133-08. View

Arena M, Russo P, Capozzi V, Lopez P, Fiocco D, Spano G . Probiotic abilities of riboflavin-overproducing Lactobacillus strains: a novel promising application of probiotics. Appl Microbiol Biotechnol. 2014; 98(17):7569-81. DOI: 10.1007/s00253-014-5837-x. View

Buck B, Azcarate-Peril M, Klaenhammer T . Role of autoinducer-2 on the adhesion ability of Lactobacillus acidophilus. J Appl Microbiol. 2009; 107(1):269-79. DOI: 10.1111/j.1365-2672.2009.04204.x. View

Sztajer H, Lemme A, Vilchez R, Schulz S, Geffers R, Yin Yip C . Autoinducer-2-regulated genes in Streptococcus mutans UA159 and global metabolic effect of the luxS mutation. J Bacteriol. 2007; 190(1):401-15. PMC: 2223724. DOI: 10.1128/JB.01086-07. View

Azcarate-Peril M, McAuliffe O, Altermann E, Lick S, Russell W, Klaenhammer T . Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus. Appl Environ Microbiol. 2005; 71(10):5794-804. PMC: 1266013. DOI: 10.1128/AEM.71.10.5794-5804.2005. View

Brinques G, do Carmo Peralba M, Ayub M . Optimization of probiotic and lactic acid production by Lactobacillus plantarum in submerged bioreactor systems. J Ind Microbiol Biotechnol. 2009; 37(2):205-12. DOI: 10.1007/s10295-009-0665-1. View

Seddik H, Bendali F, Gancel F, Fliss I, Spano G, Drider D . Lactobacillus plantarum and Its Probiotic and Food Potentialities. Probiotics Antimicrob Proteins. 2017; 9(2):111-122. DOI: 10.1007/s12602-017-9264-z. View

Badger J, Sauder J, Adams J, Antonysamy S, Bain K, Bergseid M . Structural analysis of a set of proteins resulting from a bacterial genomics project. Proteins. 2005; 60(4):787-96. DOI: 10.1002/prot.20541. View

10.

Zhang W, Wang H, Liu J, Zhao Y, Gao K, Zhang J . Adhesive ability means inhibition activities for lactobacillus against pathogens and S-layer protein plays an important role in adhesion. Anaerobe. 2013; 22:97-103. DOI: 10.1016/j.anaerobe.2013.06.005. View

11.

Fiocco D, Collins M, Muscariello L, Hols P, Kleerebezem M, Msadek T . The Lactobacillus plantarum ftsH gene is a novel member of the CtsR stress response regulon. J Bacteriol. 2008; 191(5):1688-94. PMC: 2648225. DOI: 10.1128/JB.01551-08. View

12.

Liu C, Wang R, Gong F, Liu X, Zheng H, Luo Y . Complete genome sequences and comparative genome analysis of Lactobacillus plantarum strain 5-2 isolated from fermented soybean. Genomics. 2015; 106(6):404-11. DOI: 10.1016/j.ygeno.2015.07.007. View

13.

Landete J, Arques J, Peiroten A, Langa S, Medina M . An improved method for the electrotransformation of lactic acid bacteria: A comparative survey. J Microbiol Methods. 2014; 105:130-3. DOI: 10.1016/j.mimet.2014.07.022. View

14.

Siezen R, Tzeneva V, Castioni A, Wels M, Phan H, Rademaker J . Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches. Environ Microbiol. 2009; 12(3):758-73. DOI: 10.1111/j.1462-2920.2009.02119.x. View

15.

Bosch M, Rodriguez M, Garcia F, Fernandez E, Fuentes M, Cune J . Probiotic properties of Lactobacillus plantarum CECT 7315 and CECT 7316 isolated from faeces of healthy children. Lett Appl Microbiol. 2011; 54(3):240-6. DOI: 10.1111/j.1472-765X.2011.03199.x. View

16.

de Vrese M, Schrezenmeir J . Probiotics, prebiotics, and synbiotics. Adv Biochem Eng Biotechnol. 2008; 111:1-66. DOI: 10.1007/10_2008_097. View

17.

Dirix G, Monsieurs P, Dombrecht B, Daniels R, Marchal K, Vanderleyden J . Peptide signal molecules and bacteriocins in Gram-negative bacteria: a genome-wide in silico screening for peptides containing a double-glycine leader sequence and their cognate transporters. Peptides. 2004; 25(9):1425-40. DOI: 10.1016/j.peptides.2003.10.028. View

18.

Ricciardi A, Parente E, Guidone A, Ianniello R, Zotta T, Abu Sayem S . Genotypic diversity of stress response in Lactobacillus plantarum, Lactobacillus paraplantarum and Lactobacillus pentosus. Int J Food Microbiol. 2012; 157(2):278-85. DOI: 10.1016/j.ijfoodmicro.2012.05.018. View

19.

Arena M, Capozzi V, Spano G, Fiocco D . The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms. Appl Microbiol Biotechnol. 2017; 101(7):2641-2657. DOI: 10.1007/s00253-017-8182-z. View

20.

Sun Z, He X, Brancaccio V, Yuan J, Riedel C . Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation. PLoS One. 2014; 9(2):e88260. PMC: 3914940. DOI: 10.1371/journal.pone.0088260. View