Suppression of Contractile Force in Muscle Fibers by Antibody to Myosin Subfragment 2

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Mar 1

PMID 2964637

Citations 11

Authors

S Lovell

T Karr

W F Harrington

Affiliations

Soon will be listed here.

Abstract

Polyclonal antibody directed against the subfragment-2 region of myosin was purified by affinity chromatography. Skinned muscle fibers that had been preincubated with antibody were able to sustain only 7% of the active isometric force generated by control fibers. The effect of antibody on force production could not be accounted for by inhibition of ATP turnover.

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References

Huxley H . The mechanism of muscular contraction. Science. 1969; 164(3886):1356-65. DOI: 10.1126/science.164.3886.1356. View

Porter R . The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959; 73:119-26. PMC: 1197021. DOI: 10.1042/bj0730119. View

HUXLEY A, Simmons R . Proposed mechanism of force generation in striated muscle. Nature. 1971; 233(5321):533-8. DOI: 10.1038/233533a0. View

Pepe F . Structure of muscle filaments from immunohistochemical and ultrastructural studies. J Histochem Cytochem. 1975; 23(7):543-62. DOI: 10.1177/23.7.1095653. View

Sutoh K, Karr T, Harrington W . Isolation and physico-chemical properties of a high molecular weight subfragment-2 of myosin. J Mol Biol. 1978; 126(1):1-22. DOI: 10.1016/0022-2836(78)90276-0. View

Harrington W . On the origin of the contractile force in skeletal muscle. Proc Natl Acad Sci U S A. 1979; 76(10):5066-70. PMC: 413080. DOI: 10.1073/pnas.76.10.5066. View

Gulati J, Babu A . Tonicity effects on intact single muscle fibers: relation between force and cell volume. Science. 1982; 215(4536):1109-12. DOI: 10.1126/science.6977845. View

Huxley H, Kress M . Crossbridge behaviour during muscle contraction. J Muscle Res Cell Motil. 1985; 6(2):153-61. DOI: 10.1007/BF00713057. View

Ueno H, Harrington W . Local melting in the subfragment-2 region of myosin in activated muscle and its correlation with contractile force. J Mol Biol. 1986; 190(1):69-82. DOI: 10.1016/0022-2836(86)90076-8. View

10.

Hynes T, Block S, White B, Spudich J . Movement of myosin fragments in vitro: domains involved in force production. Cell. 1987; 48(6):953-63. DOI: 10.1016/0092-8674(87)90704-5. View

11.

Harada Y, Noguchi A, Kishino A, Yanagida T . Sliding movement of single actin filaments on one-headed myosin filaments. Nature. 1987; 326(6115):805-8. DOI: 10.1038/326805a0. View

12.

Toyoshima Y, Kron S, MCNALLY E, Niebling K, Toyoshima C, Spudich J . Myosin subfragment-1 is sufficient to move actin filaments in vitro. Nature. 1987; 328(6130):536-9. DOI: 10.1038/328536a0. View

13.

Ueno H, Harrington W . Cross-linking within the thick filaments of muscle and its effect on contractile force. Biochemistry. 1987; 26(12):3589-96. DOI: 10.1021/bi00386a051. View

14.

EDMAN K, Reggiani C . The sarcomere length-tension relation determined in short segments of intact muscle fibres of the frog. J Physiol. 1987; 385:709-32. PMC: 1192369. DOI: 10.1113/jphysiol.1987.sp016516. View

15.

Harrington W . A mechanochemical mechanism for muscle contraction. Proc Natl Acad Sci U S A. 1971; 68(3):685-9. PMC: 389017. DOI: 10.1073/pnas.68.3.685. View