Probiotic Mixture of Lactobacillus and Bifidobacterium Alleviates Systemic Adiposity and Inflammation in Non-alcoholic Fatty Liver Disease Rats Through Gpr109a and the Commensal Metabolite Butyrate

Overview

Journal Inflammopharmacology

Specialty Pharmacology

Date 2018 Apr 11

PMID 29633106

Citations 24

Authors

Yinji Liang

Chenli Lin

Yupei Zhang

Yuanjun Deng

Chan Liu

Qinhe Yang

Affiliations

Soon will be listed here.

Abstract

Aims: The study explored the systemic adiposity and inflammation through Gpr109a and the commensal metabolite butyrate during the treatment of non-alcoholic fatty liver disease rats with the probiotic mixture of Lactobacillus and Bifidobacterium for 16 weeks.

Methods: Fifteen male SD rats were randomly divided into three groups of five rats each: normal control group (basal feed), high-fat diet (HFD) feeding group (83% basal feed + 10% lard oil + 5% sucrose + 1.5% cholesterol + 0.5% cholate), and probiotic mixture intervention group (HFD + 0.6 g kg day probiotic mixture). Body composition, serum lipids, serum inflammatory markers, Gpr109a, and the commensal metabolite butyrate were assessed.

Results: Compared with HFD group, probiotic mixture significantly reduced body weight and the levels of serum FFA, TG, ALT, IL-1β, and IL-18 (P < 0.05). The levels of Gpr109a and the commensal metabolite butyrate also changed significantly (P < 0.05).

Conclusions: Probiotic mixture might inhibit systemic adiposity and inflammation through Gpr109a and the commensal metabolite butyrate in response to the insult of HFD.

Citing Articles

The role of intestinal flora in metabolic dysfunction-associated steatotic liver disease and treatment strategies.

Wang L, Sun J, Chen S, Sun Y, Zheng Y, Feng J Front Med (Lausanne). 2025; 11():1490929.

PMID: 39839647 PMC: 11746088. DOI: 10.3389/fmed.2024.1490929.

Meta-analysis of the effects of probiotics on hyperlipidemia.

Yao Y, Hong Q, Ding S, Cui J, Li W, Zhang J Curr Res Food Sci. 2024; 9:100885.

PMID: 39469722 PMC: 11513789. DOI: 10.1016/j.crfs.2024.100885.

Fecal fermentation behaviors of Konjac glucomannan and its impacts on human gut microbiota.

Tan X, Wang B, Zhou X, Liu C, Wang C, Bai J Food Chem X. 2024; 23:101610.

PMID: 39071938 PMC: 11282934. DOI: 10.1016/j.fochx.2024.101610.

The impact of dietary fructose on gut permeability, microbiota, abdominal adiposity, insulin signaling and reproductive function.

Guney C, Bal N, Akar F Heliyon. 2023; 9(8):e18896.

PMID: 37636431 PMC: 10447940. DOI: 10.1016/j.heliyon.2023.e18896.

Probiotics, prebiotics, and synbiotics in nonalcoholic fatty liver disease and alcohol-associated liver disease.

Kaufmann B, Seyfried N, Hartmann D, Hartmann P Am J Physiol Gastrointest Liver Physiol. 2023; 325(1):G42-G61.

PMID: 37129252 PMC: 10312326. DOI: 10.1152/ajpgi.00017.2023.

References

Li Z, Li X, Lin S, Chen Y, Ma S, Fu Y . Nicotinic Acid Receptor GPR109A Exerts Anti-Inflammatory Effects Through Inhibiting the Akt/mTOR Signaling Pathway in MIN6 Pancreatic β cells. Ann Clin Lab Sci. 2017; 47(6):729-737. View

Koh A, De Vadder F, Kovatcheva-Datchary P, Backhed F . From Dietary Fiber to Host Physiology: Short-Chain Fatty Acids as Key Bacterial Metabolites. Cell. 2016; 165(6):1332-1345. DOI: 10.1016/j.cell.2016.05.041. View

Zhao G, Nyman M, Jonsson J . Rapid determination of short-chain fatty acids in colonic contents and faeces of humans and rats by acidified water-extraction and direct-injection gas chromatography. Biomed Chromatogr. 2005; 20(8):674-82. DOI: 10.1002/bmc.580. View

Tunaru S, Kero J, Schaub A, Wufka C, Blaukat A, Pfeffer K . PUMA-G and HM74 are receptors for nicotinic acid and mediate its anti-lipolytic effect. Nat Med. 2003; 9(3):352-5. DOI: 10.1038/nm824. View

Endo H, Niioka M, Kobayashi N, Tanaka M, Watanabe T . Butyrate-producing probiotics reduce nonalcoholic fatty liver disease progression in rats: new insight into the probiotics for the gut-liver axis. PLoS One. 2013; 8(5):e63388. PMC: 3656030. DOI: 10.1371/journal.pone.0063388. View

Liang Y, Liang S, Zhang Y, Deng Y, He Y, Chen Y . Oral Administration of Compound Probiotics Ameliorates HFD-Induced Gut Microbe Dysbiosis and Chronic Metabolic Inflammation via the G Protein-Coupled Receptor 43 in Non-alcoholic Fatty Liver Disease Rats. Probiotics Antimicrob Proteins. 2018; 11(1):175-185. DOI: 10.1007/s12602-017-9378-3. View

Zechner R, Strauss J, Haemmerle G, Lass A, Zimmermann R . Lipolysis: pathway under construction. Curr Opin Lipidol. 2005; 16(3):333-40. DOI: 10.1097/01.mol.0000169354.20395.1c. View

Lu Y, Fan C, Li P, Lu Y, Chang X, Qi K . Short Chain Fatty Acids Prevent High-fat-diet-induced Obesity in Mice by Regulating G Protein-coupled Receptors and Gut Microbiota. Sci Rep. 2016; 6:37589. PMC: 5124860. DOI: 10.1038/srep37589. View

Feingold K, Moser A, Shigenaga J, Grunfeld C . Inflammation stimulates niacin receptor (GPR109A/HCA2) expression in adipose tissue and macrophages. J Lipid Res. 2014; 55(12):2501-8. PMC: 4242443. DOI: 10.1194/jlr.M050955. View

10.

Wang H, Li S, Zhang Y, Guan J, Wu Y, Wang Q . Therapeutic efficacy and mechanism of Zhenrenyangzang decoction in rats with experimental ulcerative colitis. Int J Clin Exp Med. 2015; 8(9):15254-61. PMC: 4658900. View

11.

Xu X, Lin S, Chen Y, Li X, Ma S, Fu Y . The Effect of Metformin on the Expression of GPR109A, NF-κB and IL-1β in Peripheral Blood Leukocytes from Patients with Type 2 Diabetes Mellitus. Ann Clin Lab Sci. 2017; 47(5):556-562. View