Rapidly Responsive Silk Fibroin Hydrogels As an Artificial Matrix for the Programmed Tumor Cells Death

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 Apr 5

PMID 29617395

Citations 24

Authors

Viviana P Ribeiro

Joana Silva-Correia

Cristiana Goncalves

Sandra Pina

Hajer Radhouani

Toni Montonen

Jari Hyttinen

Anirban Roy

Ana L Oliveira

Rui L Reis

Joaquim M Oliveira

Affiliations

Soon will be listed here.

Abstract

Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix. The proposed SF hydrogels presented a viscoelastic nature of injectable hydrogels and spontaneous conformational changes from random coil to β-sheet conformation under physiological conditions. A human neuronal glioblastoma (U251) cell line was used for screening cell encapsulation and in vitro evaluation within the SF hydrogels. The transparent random coil SF hydrogels promoted cell viability and proliferation up to 10 days of culturing, while the crystalline SF hydrogels converted into β-sheet structure induced the formation of TUNEL-positive apoptotic cells. Therefore, this work provides a powerful tool for the investigation of the microenvironment on the programed tumor cells death, by using rapidly responsive SF hydrogels as 3D in vitro tumor models.

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