Physicochemical Study of Natural Fractionated Biocolloid by Asymmetric Flow Field-flow Fractionation in Tandem with Various Complementary Techniques Using Biologically Synthesized Silver Nanocomposites

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2018 Apr 5

PMID 29616293

Citations 6

Authors

Viorica Railean-Plugaru

Pawel Pomastowski

Tomasz Kowalkowski

Myroslav Sprynskyy

Boguslaw Buszewski

Affiliations

Soon will be listed here.

Abstract

Asymmetric flow field-flow fractionation coupled with use of ultraviolet-visible, multiangle light scattering (MALLS), and dynamic light scattering (DLS) detectors was used for separation and characterization of biologically synthesized silver composites in two liquid compositions. Moreover, to supplement the DLS/MALLS information, various complementary techniques such as transmission electron spectroscopy, Fourier transform infrared spectroscopy, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used. The hydrodynamic diameter and the radius of gyration of silver composites were slightly larger than the sizes obtained by transmission electron microscopy (TEM). Moreover, the TEM results revealed the presence of silver clusters and even several morphologies, including multitwinned. Additionally, MALDI-TOF MS examination showed that the particles have an uncommon cluster structure. It can be described as being composed of two or more silver clusters. The organic surface of the nanoparticles can modify their dispersion. We demonstrated that the variation of the silver surface coating directly influenced the migration rate of biologically synthesized silver composites. Moreover, this study proves that the fractionation mechanism of silver biocolloids relies not only on the particle size but also on the type and mass of the surface coatings. Because silver nanoparticles typically have size-dependent cytotoxicity, this behavior is particularly relevant for biomedical applications. Graphical abstract Workflow for asymmetric flow field-flow fractionation of natural biologically synthesized silver nanocomposites.

Citing Articles

Field-Flow Fractionation in Molecular Biology and Biotechnology.

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The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach.

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Zinc Oxide Nanocomposites-Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential.

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The Influence of Different Forms of Silver on Selected Pathogenic Bacteria.

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