» Articles » PMID: 29609310

Computational Localization Microscopy with Extended Axial Range

Overview
Journal Opt Express
Date 2018 Apr 4
PMID 29609310
Citations 3
Authors
Affiliations
Soon will be listed here.
Abstract

A new single-aperture 3D particle-localization and tracking technique is presented that demonstrates an increase in depth range by more than an order of magnitude without compromising optical resolution and throughput. We exploit the extended depth range and depth-dependent translation of an Airy-beam PSF for 3D localization over an extended volume in a single snapshot. The technique is applicable to all bright-field and fluorescence modalities for particle localization and tracking, ranging from super-resolution microscopy through to the tracking of fluorescent beads and endogenous particles within cells. We demonstrate and validate its application to real-time 3D velocity imaging of fluid flow in capillaries using fluorescent tracer beads. An axial localization precision of 50 nm was obtained over a depth range of 120μm using a 0.4NA, 20× microscope objective. We believe this to be the highest ratio of axial range-to-precision reported to date.

Citing Articles

Optimizing self-interference digital holography for single-molecule localization.

Li S, Kner P Opt Express. 2023; 31(18):29352-29367.

PMID: 37710737 PMC: 10544951. DOI: 10.1364/OE.499724.


Holistic Monte-Carlo optical modelling of biological imaging.

Carles G, Zammit P, Harvey A Sci Rep. 2019; 9(1):15832.

PMID: 31676825 PMC: 6825179. DOI: 10.1038/s41598-019-51850-1.


High-speed extended-volume blood flow measurement using engineered point-spread function.

Zhou Y, Zickus V, Zammit P, Taylor J, Harvey A Biomed Opt Express. 2019; 9(12):6444-6454.

PMID: 31065441 PMC: 6490974. DOI: 10.1364/BOE.9.006444.