In Vivo EPR Assessment of PH, PO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Overview

Journal J Vis Exp

Specialties Biology
General Medicine

Date 2018 Apr 3

PMID 29608148

Citations 12

Authors

Andrey A Bobko

Timothy D Eubank

Benoit Driesschaert

Valery V Khramtsov

Affiliations

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Abstract

This protocol demonstrates the capability of low-field electron paramagnetic resonance (EPR)-based techniques in combination with functional paramagnetic probes to provide quantitative information on the chemical tumor microenvironment (TME), including pO2, pH, redox status, concentrations of interstitial inorganic phosphate (Pi), and intracellular glutathione (GSH). In particular, an application of a recently developed soluble multifunctional trityl probe provides unsurpassed opportunity for in vivo concurrent measurements of pH, pO2 and Pi in Extracellular space (HOPE probe). The measurements of three parameters using a single probe allow for their correlation analyses independent of probe distribution and time of the measurements.

Citing Articles

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Electron Spin Resonance Probe Incorporation into Bioinks Permits Longitudinal Oxygen Imaging of Bioprinted Constructs.

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In Vivo Electron Paramagnetic Resonance Molecular Profiling of Tumor Microenvironment upon Tumor Progression to Malignancy in an Animal Model of Breast Cancer.

Eubank T, Bobko A, Hoblitzell E, Gencheva M, Driesschaert B, Khramtsov V Mol Imaging Biol. 2023; 26(3):424-434.

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Improving combination therapies: targeting A2B-adenosine receptor to modulate metabolic tumor microenvironment and immunosuppression.

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