Pretreatment with Shuanghe-Tang Extract Attenuates Postischemic Brain Injury and Edema in a Mouse Model of Stroke: An Analysis of Medicinal Herbs Listed in

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2018 Mar 31

PMID 29599893

Citations 3

Authors

Min Jae Kim

Seo-Yeon Lee

Ji Young Hwang

Hyunha Kim

Ki-Tae Ha

Byung Tae Choi

Jin Ung Baek

Hwa Kyoung Shin

Affiliations

Soon will be listed here.

Abstract

Aim: Although stroke is among the leading causes of death and long-term disability, there are few effective treatments for limiting the severity of neurological sequelae. We evaluated the effects of 29 medicinal herbs listed in the chapter of the 17th century Korean medical text on stroke symptoms in a mouse model of cerebral ischemia.

Methods: Focal cerebral ischemia was induced via photothrombosis. Infarct volume, brain edema, and neurological deficits were evaluated. Immunofluorescence staining for tight junction proteins and aquaporin 4 (AQP4) was performed following ischemic injury.

Results: Based on our initial findings, we examined the effects of two prescriptions in which the candidate herbs comprised more than 60% of the total formula: Shuanghe-tang and Zengsunsiwu-tang. Pretreatment with Shuanghe-tang significantly reduced infarct volume, decreased blood-brain barrier (BBB) breakdown, attenuated edema, and improved neurological and motor functions in a dose-dependent manner (30, 100, and 300 mg/kg), while no such effects were observed in mice pretreated with Zengsunsiwu-tang. Immunohistochemical analysis revealed significant increases in ipsilateral occludin and zonula occludens 1 (ZO-1) expression in Shuanghe-tang-pretreated mice, as well as increased AQP4 immunofluorescence.

Conclusions: These results indicate that Shuanghe-tang may protect against brain injury and promote recovery of neurological function following ischemia.

Citing Articles

Benefits of a Skull-Interfaced Flexible and Implantable Multilight Emitting Diode Array for Photobiomodulation in Ischemic Stroke.

Kim H, Kim M, Kwon Y, Jeon S, Lee S, Kim C Adv Sci (Weinh). 2022; 9(11):e2104629.

PMID: 35076161 PMC: 9008794. DOI: 10.1002/advs.202104629.

Weisheng-Tang Ameliorates Acute Ischemic Brain Damage in Mice by Maintaining Blood-Brain Barrier Integrity.

Kim M, Park K, Lee J, Ha K, Choi B, Baek J Oxid Med Cell Longev. 2019; 2019:4379732.

PMID: 31885791 PMC: 6914926. DOI: 10.1155/2019/4379732.

Anfibatide Preserves Blood-Brain Barrier Integrity by Inhibiting TLR4/RhoA/ROCK Pathway After Cerebral Ischemia/Reperfusion Injury in Rat.

Gong P, Li R, Jia H, Ma Z, Li X, Dai X J Mol Neurosci. 2019; 70(1):71-83.

PMID: 31478134 DOI: 10.1007/s12031-019-01402-z.

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