Dissociation of NS5 from Cell Fractions Containing West Nile Virus-specific Polymerase Activity

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1987 Nov 1

PMID 2959795

Citations 28

Authors

J B Grun

M A Brinton

Affiliations

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Abstract

West Nile virus replication complexes were partially purified from cytoplasmic extracts of virus-infected cells by centrifugation through a 20% glycerol cushion. Numerous cell proteins, as well as the largest nonstructural protein, NS5, were separated from the replication complexes without significant loss of in vitro West Nile virus polymerase activity.

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