Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A As an Intermediate Layer

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2018 Mar 31

PMID 29597326

Citations 6

Authors

Raffaele Caroselli

Javier Garcia Castello

Jorge Escorihuela

Maria Jose Banuls

Angel Maquieira

Jaime Garcia-Ruperez

Affiliations

Soon will be listed here.

Abstract

A proper antibody immobilization on a biosensor is a crucial step in order to obtain a high sensitivity to be able to detect low target analyte concentrations. In this paper, we present an experimental study of the immobilization process of antibodies as bioreceptors on a photonic ring resonator sensor. A protein A intermediate layer was created on the sensor surface in order to obtain an oriented immobilization of the antibodies, which enhances the interaction with the target antigens to be detected. The anti-bovine serum albumin (antiBSA)-bovine serum albumin (BSA) pair was used as a model for our study. An opto-fluidic setup was developed in order to flow the different reagents and, simultaneously, to monitor in real-time the spectral response of the photonic sensing structure. The antiBSA immobilization and the BSA detection, their repeatability, and specificity were studied in different conditions of the sensor surface. Finally, an experimental limit of detection for BSA recognition of only 1 ng/mL was obtained.

Citing Articles

Silicon Microring Resonator Biosensor for Detection of Nucleocapsid Protein of SARS-CoV-2.

Uchida Y, Arakawa T, Higo A, Ishizaka Y Sensors (Basel). 2024; 24(10).

PMID: 38794104 PMC: 11125905. DOI: 10.3390/s24103250.

Biosensors Based on Stanniocalcin-1 Protein Antibodies Thin Films for Prostate Cancer Diagnosis.

Ferreira R, Ribeiro P, Canario A, Raposo M Biosensors (Basel). 2023; 13(11).

PMID: 37998156 PMC: 10669463. DOI: 10.3390/bios13110981.

Performance improvement of a silicon nitride ring resonator biosensor operated in the TM mode at 1310 nm.

Castello-Pedrero L, Gomez-Gomez M, Garcia-Ruperez J, Griol A, Martinez A Biomed Opt Express. 2021; 12(11):7244-7260.

PMID: 34858712 PMC: 8606153. DOI: 10.1364/BOE.437823.

Advances in Electrochemical and Acoustic Aptamer-Based Biosensors and Immunosensors in Diagnostics of Leukemia.

Hianik T Biosensors (Basel). 2021; 11(6).

PMID: 34073054 PMC: 8227535. DOI: 10.3390/bios11060177.

Porous Silicon Optical Devices: Recent Advances in Biosensing Applications.

Moretta R, De Stefano L, Terracciano M, Rea I Sensors (Basel). 2021; 21(4).

PMID: 33668616 PMC: 7917735. DOI: 10.3390/s21041336.

References

Scullion M, Krauss T, Di Falco A . Slotted photonic crystal sensors. Sensors (Basel). 2013; 13(3):3675-710. PMC: 3658769. DOI: 10.3390/s130303675. View

Wang Z, Jin G . Feasibility of protein A for the oriented immobilization of immunoglobulin on silicon surface for a biosensor with imaging ellipsometry. J Biochem Biophys Methods. 2003; 57(3):203-11. DOI: 10.1016/s0165-022x(03)00109-x. View

Fernandez Gavela A, Grajales Garcia D, C Ramirez J, Lechuga L . Last Advances in Silicon-Based Optical Biosensors. Sensors (Basel). 2016; 16(3):285. PMC: 4813860. DOI: 10.3390/s16030285. View

Castello J, Toccafondo V, Perez-Millan P, Losilla N, Cruz J, Andres M . Real-time and low-cost sensing technique based on photonic bandgap structures. Opt Lett. 2011; 36(14):2707-9. DOI: 10.1364/OL.36.002707. View

Shang J, Cheng F, Dubey M, Kaplan J, Rawal M, Jiang X . An organophosphonate strategy for functionalizing silicon photonic biosensors. Langmuir. 2012; 28(6):3338-44. PMC: 3291497. DOI: 10.1021/la2043153. View

Jonkheijm P, Weinrich D, Schroder H, Niemeyer C, Waldmann H . Chemical strategies for generating protein biochips. Angew Chem Int Ed Engl. 2008; 47(50):9618-47. DOI: 10.1002/anie.200801711. View

Peter B, Farkas E, Forgacs E, Saftics A, Kovacs B, Kurunczi S . Green tea polyphenol tailors cell adhesivity of RGD displaying surfaces: multicomponent models monitored optically. Sci Rep. 2017; 7:42220. PMC: 5301484. DOI: 10.1038/srep42220. View

Lee M, Fauchet P . Two-dimensional silicon photonic crystal based biosensing platform for protein detection. Opt Express. 2009; 15(8):4530-5. DOI: 10.1364/oe.15.004530. View

Julian E, Cama M, Martinez P, Luquin M . An ELISA for five glycolipids from the cell wall of Mycobacterium tuberculosis: Tween 20 interference in the assay. J Immunol Methods. 2001; 251(1-2):21-30. DOI: 10.1016/s0022-1759(01)00313-1. View

10.

Luchansky M, Washburn A, Martin T, Iqbal M, Gunn L, Bailey R . Characterization of the evanescent field profile and bound mass sensitivity of a label-free silicon photonic microring resonator biosensing platform. Biosens Bioelectron. 2010; 26(4):1283-91. PMC: 2997171. DOI: 10.1016/j.bios.2010.07.010. View

11.

Sipova H, Zhang S, Dudley A, Galas D, Wang K, Homola J . Surface plasmon resonance biosensor for rapid label-free detection of microribonucleic acid at subfemtomole level. Anal Chem. 2010; 82(24):10110-5. PMC: 3021551. DOI: 10.1021/ac102131s. View

12.

Escorihuela J, Banuls M, Garcia Castello J, Toccafondo V, Garcia-Ruperez J, Puchades R . Chemical silicon surface modification and bioreceptor attachment to develop competitive integrated photonic biosensors. Anal Bioanal Chem. 2012; 404(10):2831-40. DOI: 10.1007/s00216-012-6280-4. View

13.

Vollmer F, Yang L . Label-free detection with high-Q microcavities: a review of biosensing mechanisms for integrated devices. Nanophotonics. 2016; 1(3-4):267-291. PMC: 4764104. DOI: 10.1515/nanoph-2012-0021. View

14.

Banuls M, Puchades R, Maquieira A . Chemical surface modifications for the development of silicon-based label-free integrated optical (IO) biosensors: a review. Anal Chim Acta. 2013; 777:1-16. DOI: 10.1016/j.aca.2013.01.025. View

15.

Buswell S, Wright V, Buriak J, Van V, Evoy S . Specific detection of proteins using photonic crystal waveguides. Opt Express. 2008; 16(20):15949-57. DOI: 10.1364/oe.16.015949. View

16.

Anderson G, Jacoby M, Ligler F, King K . Effectiveness of protein A for antibody immobilization for a fiber optic biosensor. Biosens Bioelectron. 1997; 12(4):329-36. DOI: 10.1016/s0956-5663(96)00074-7. View

17.

Fan X, White I, Shopova S, Zhu H, Suter J, Sun Y . Sensitive optical biosensors for unlabeled targets: a review. Anal Chim Acta. 2008; 620(1-2):8-26. PMC: 10069299. DOI: 10.1016/j.aca.2008.05.022. View

18.

Garcia-Ruperez J, Toccafondo V, Banuls M, Garcia Castello J, Griol A, Peransi-Llopis S . Label-free antibody detection using band edge fringes in SOI planar photonic crystal waveguides in the slow-light regime. Opt Express. 2010; 18(23):24276-86. DOI: 10.1364/OE.18.024276. View

19.

Xu D, Vachon M, Densmore A, Ma R, Delage A, Janz S . Label-free biosensor array based on silicon-on-insulator ring resonators addressed using a WDM approach. Opt Lett. 2010; 35(16):2771-3. DOI: 10.1364/OL.35.002771. View

20.

Rusmini F, Zhong Z, Feijen J . Protein immobilization strategies for protein biochips. Biomacromolecules. 2007; 8(6):1775-89. DOI: 10.1021/bm061197b. View