Ectopic Overexpression of a Novel R2R3-MYB, NtMYB2 from Chinese Narcissus Represses Anthocyanin Biosynthesis in Tobacco

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2018 Mar 31

PMID 29597321

Citations 23

Authors

Muhammad Anwar

Guiqing Wang

Jiacheng Wu

Saquib Waheed

Andrew C Allan

Lihui Zeng

Affiliations

Soon will be listed here.

Abstract

R2R3 MYB transcription factors play key functions in the regulation of secondary metabolites. In the present study, a R2R3 MYB transcriptional factor was identified from Chinese narcissus ( L. var. Roem) and functionally characterized. belongs to subgroup 4 of the R2R3 MYB transcription factor family that are related to repressor MYBs involved in the regulation of anthocyanin and flavonoids. Transient expression confirmed that strongly reduced the red pigmentation induced by MYB- anthocyanin activators in agro-infiltrated tobacco leaves. Ectopic expression of in tobacco significantly reduced the pigmentation and altered the floral phenotypes in transgenic tobacco flowers. Gene expression analysis suggested that repressed the transcript levels of structural genes involved in anthocyanin biosynthesis pathway, especially the gene. gene is expressed in all examined narcissus tissues; the levels of transcription in petals and corona is higher than other tissues and the transcription level at the bud stage was highest. These results show that is involved in the regulation of anthocyanin biosynthesis pathway and may act as a repressor by down regulating the transcripts of key enzyme genes in Chinese narcissus.

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