Biofunctionalized Scaffold in Bone Tissue Repair

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2018 Mar 30

PMID 29596323

Citations 41

Authors

Francesca Diomede

Marco dAurora

Agnese Gugliandolo

Ilaria Merciaro

Tiziana Orsini

Valentina Gatta

Adriano Piattelli

Oriana Trubiani

Emanuela Mazzon

Affiliations

Soon will be listed here.

Abstract

Bone tissue engineering is based on bone grafting to repair bone defects. Bone graft substitutes can contribute to the addition of mesenchymal stem cells (MSCs) in order to enhance the rate and the quality of defect regeneration. The stem cell secretome contains many growth factors and chemokines, which could affect cellular characteristics and behavior. Conditioned medium (CM) could be used in tissue regeneration avoiding several problems linked to the direct use of MSCs. In this study, we investigated the effect of human periodontal ligament stem cells (hPDLSCs) and their CM on bone regeneration using a commercially available membrane scaffold Evolution (EVO) implanted in rat calvarias. EVO alone or EVO + hPDLSCs with or without CM were implanted in Wistar male rats subjected to calvarial defects. The in vivo results revealed that EVO membrane enriched with hPDLSCs and CM showed a better osteogenic ability to repair the calvarial defect. These results were confirmed by acquired micro-computed tomography (CT) images and the increased osteopontin levels. Moreover, RT-PCR in vitro revealed the upregulation of three genes (Collagen (), and transforming growth factor ()) and the down regulation of 26 genes involved in bone regeneration. These results suggest a promising potential application of CM from hPDLSCs and scaffolds for bone defect restoration and in particular for calvarial repair in case of trauma.

Citing Articles

Revolutionizing bone defect healing: the power of mesenchymal stem cells as seeds.

Zhang Y, Fan M, Zhang Y Front Bioeng Biotechnol. 2024; 12:1421674.

PMID: 39497791 PMC: 11532096. DOI: 10.3389/fbioe.2024.1421674.

Comparative assessment of the physical structure of antler and bovine bone substitutes: An in vitro study.

Mahboubian M, Kadkhodazadeh M, Amid R, Moscowchi A J Adv Periodontol Implant Dent. 2024; 16(1):4-8.

PMID: 39027212 PMC: 11252153. DOI: 10.34172/japid.2024.012.

Transcriptomic and proteomic strategies to reveal the mechanism of Gymnocypris przewalskii scale development.

Xu B, Cui Y, A L, Zhang H, Ma Q, Wei F BMC Genomics. 2024; 25(1):140.

PMID: 38310220 PMC: 10837935. DOI: 10.1186/s12864-024-10047-1.

Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue.

Abbadessa A, Ronca A, Salerno A Drug Deliv Transl Res. 2023; 14(4):858-894.

PMID: 37882983 PMC: 10927859. DOI: 10.1007/s13346-023-01437-1.

Metal-organic frameworks functionalized biomaterials for promoting bone repair.

Zhao C, Shu C, Yu J, Zhu Y Mater Today Bio. 2023; 21:100717.

PMID: 37545559 PMC: 10401359. DOI: 10.1016/j.mtbio.2023.100717.

References

Zhang C, Yan B, Cui Z, Cui S, Zhang T, Wang X . Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells. Sci Rep. 2017; 7(1):10519. PMC: 5585269. DOI: 10.1038/s41598-017-11155-7. View

Ballerini P, Diomede F, Petragnani N, Cicchitti S, Merciaro I, Cavalcanti M . Conditioned medium from relapsing-remitting multiple sclerosis patients reduces the expression and release of inflammatory cytokines induced by LPS-gingivalis in THP-1 and MO3.13 cell lines. Cytokine. 2017; 96:261-272. DOI: 10.1016/j.cyto.2017.04.022. View

Diomede F, Rajan T, Gatta V, dAurora M, Merciaro I, Marchisio M . Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage. Stem Cells Int. 2017; 2017:5651287. PMC: 5392399. DOI: 10.1155/2017/5651287. View

Ekwueme E, Shah J, Mohiuddin M, Ghebes C, Crispim J, Saris D . Cross-Talk Between Human Tenocytes and Bone Marrow Stromal Cells Potentiates Extracellular Matrix Remodeling In Vitro. J Cell Biochem. 2015; 117(3):684-93. PMC: 4943840. DOI: 10.1002/jcb.25353. View

Gao C, Peng S, Feng P, Shuai C . Bone biomaterials and interactions with stem cells. Bone Res. 2017; 5:17059. PMC: 5738879. DOI: 10.1038/boneres.2017.59. View

Shakir S, MacIsaac Z, Naran S, Smith D, Bykowski M, Cray J . Transforming growth factor beta 1 augments calvarial defect healing and promotes suture regeneration. Tissue Eng Part A. 2014; 21(5-6):939-47. PMC: 4356478. DOI: 10.1089/ten.TEA.2014.0189. View

Vanecek V, Klima K, Kohout A, Foltan R, Jirousek O, Sedy J . The combination of mesenchymal stem cells and a bone scaffold in the treatment of vertebral body defects. Eur Spine J. 2013; 22(12):2777-86. PMC: 3843808. DOI: 10.1007/s00586-013-2991-2. View

Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M . Tissue-engineered bone regeneration. Nat Biotechnol. 2000; 18(9):959-63. DOI: 10.1038/79449. View

Otero L, Carrillo N, Calvo-Guirado J, Villamil J, Delgado-Ruiz R . Osteogenic potential of platelet-rich plasma in dental stem-cell cultures. Br J Oral Maxillofac Surg. 2017; 55(7):697-702. DOI: 10.1016/j.bjoms.2017.05.005. View

10.

Zhao L, Jiang S, Hantash B . Transforming growth factor beta1 induces osteogenic differentiation of murine bone marrow stromal cells. Tissue Eng Part A. 2009; 16(2):725-33. DOI: 10.1089/ten.TEA.2009.0495. View

11.

Kawai T, Katagiri W, Osugi M, Sugimura Y, Hibi H, Ueda M . Secretomes from bone marrow-derived mesenchymal stromal cells enhance periodontal tissue regeneration. Cytotherapy. 2015; 17(4):369-81. DOI: 10.1016/j.jcyt.2014.11.009. View

12.

Baik H, Park J, Lee K, Chung C . Local application of periodontal ligament stromal cells promotes soft tissue regeneration. Oral Dis. 2013; 20(6):574-81. DOI: 10.1111/odi.12175. View

13.

Moshaverinia A, Chen C, Xu X, Akiyama K, Ansari S, Zadeh H . Bone regeneration potential of stem cells derived from periodontal ligament or gingival tissue sources encapsulated in RGD-modified alginate scaffold. Tissue Eng Part A. 2013; 20(3-4):611-21. PMC: 3926152. DOI: 10.1089/ten.TEA.2013.0229. View

14.

Cobo T, Viloria C, Solares L, Fontanil T, Gonzalez-Chamorro E, de Carlos F . Role of Periostin in Adhesion and Migration of Bone Remodeling Cells. PLoS One. 2016; 11(1):e0147837. PMC: 4725750. DOI: 10.1371/journal.pone.0147837. View

15.

Zhang Q, Chen B, Yan F, Guo J, Zhu X, Ma S . Interleukin-10 inhibits bone resorption: a potential therapeutic strategy in periodontitis and other bone loss diseases. Biomed Res Int. 2014; 2014:284836. PMC: 3947664. DOI: 10.1155/2014/284836. View

16.

Orciani M, Trubiani O, Guarnieri S, Ferrero E, Di Primio R . CD38 is constitutively expressed in the nucleus of human hematopoietic cells. J Cell Biochem. 2008; 105(3):905-12. DOI: 10.1002/jcb.21887. View

17.

Park J, Shim J, Choi S, Jang J, Kim M, Lee S . 3D printing technology to control BMP-2 and VEGF delivery spatially and temporally to promote large-volume bone regeneration. J Mater Chem B. 2020; 3(27):5415-5425. DOI: 10.1039/c5tb00637f. View

18.

Rajan T, Giacoppo S, Diomede F, Ballerini P, Paolantonio M, Marchisio M . The secretome of periodontal ligament stem cells from MS patients protects against EAE. Sci Rep. 2016; 6:38743. PMC: 5141419. DOI: 10.1038/srep38743. View

19.

Cianci E, Recchiuti A, Trubiani O, Diomede F, Marchisio M, Miscia S . Human Periodontal Stem Cells Release Specialized Proresolving Mediators and Carry Immunomodulatory and Prohealing Properties Regulated by Lipoxins. Stem Cells Transl Med. 2015; 5(1):20-32. PMC: 4704879. DOI: 10.5966/sctm.2015-0163. View

20.

Kalfas I . Principles of bone healing. Neurosurg Focus. 2006; 10(4):E1. DOI: 10.3171/foc.2001.10.4.2. View