Slow Infusion of Recombinant Adeno-Associated Viruses into the Mouse Cerebrospinal Fluid Space

Overview

Journal Hum Gene Ther Methods

Publisher Mary Ann Liebert

Specialties Genetics
Pharmacology

Date 2018 Mar 30

PMID 29596011

Citations 5

Authors

Dan Wang

Jia Li

Karen Tran

Daniel R Burt

Li Zhong

Guangping Gao

Affiliations

Soon will be listed here.

Abstract

Recombinant adeno-associated viruses (rAAVs) are the leading in vivo gene delivery platform, and have been extensively studied in gene therapy targeting various tissues, including the central nervous system (CNS). A single-bolus rAAV injection to the cerebrospinal fluid (CSF) space has been widely used to target the CNS, but it suffers from several drawbacks, such as leakage to peripheral tissues. Here, a protocol is described using an osmotic pump to infuse rAAV slowly into the mouse CSF space. Compared to the single-bolus injection technique, pump infusion can lead to higher CNS transduction and lower transduction in the peripheral tissues.

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