Selection of DNA Aptamers Against Mycobacterium Tuberculosis Ag85A, and Its Application in a Graphene Oxide-based Fluorometric Assay

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2018 Mar 30

PMID 29594592

Citations 12

Authors

Najmeh Ansari

Kiarash Ghazvini

Mohammad Ramezani

Mahin Shahdordizadeh

Rezvan Yazdian-Robati

Khalil Abnous

Seyed Mohammad Taghdisi

Affiliations

Soon will be listed here.

Abstract

The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. It is a potential marker for early diagnosis of tuberculosis (TB). The authors have identified specific aptamers for Ag85A (FbpA) via protein SELEX using magnetic beads. After twelve rounds of selection, two aptamers (Apt8 and Apt22) were chosen from different groups, and their binding constants were determined by flow cytometry. Apt22 (labeled with Atto 647N) binds to FbpA with high affinity (K = 63 nM) and specificity. A rapid, sensitive, and low-cost fluorescent assay was designed based on the use of Apt22 and graphene oxide, with a limit of detection of 1.5 nM and an analytical range from 5 to 200 nM of FbpA. Graphical abstract Schematic illustration of graphene oxide-based aptasensor for fluorometric determination of FbpA.

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