Glucose Oxidase Assisted Visual Detection of Glucose Using Oxygen Deficient α-MoO Nanoflakes

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2018 Mar 30

PMID 29594588

Citations 5

Authors

Yowan Nerthigan

Amit Kumar Sharma

Sunil Pandey

Krishna Hari Sharma

M Shahnawaz Khan

Da-Ren Hang

Hui-Fen Wu

Affiliations

Soon will be listed here.

Abstract

An optical method is described for the quantitation of glucose by using oxygen-deficient α-MoO nanoflakes. It is based on the use of glucose oxidase (GOx) which produces hydrogen peroxide on oxidation of glucose. Hydrogen peroxide then oxidizes the α-MoO nanoflakes, and this results in a visible color change from blue to colorless. The color change can be measured photometrically at 740 nm. The method has a 68 nM detection limit. Graphical Abstract Mechanism of glucose detection using blue colored oxygen deficient 2D α-MoO nanoflakes. Hydrogen peroxide (HO) is formed as a by-product in the conversion of glucose to glucono-1,5-lactone by glucose oxidase (GO). In the presence of HO, the oxygen vacancies in α-MoO nanoflakes are filled up, and this leads to the loss of blue color of the nanoflakes because they are converted back to colorless bulk α-MoO.

Citing Articles

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References

Dong Y, Zhang H, Rahman Z, Su L, Chen X, Hu J . Graphene oxide-Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose. Nanoscale. 2012; 4(13):3969-76. DOI: 10.1039/c2nr12109c. View

Heller A, Feldman B . Electrochemical glucose sensors and their applications in diabetes management. Chem Rev. 2008; 108(7):2482-505. DOI: 10.1021/cr068069y. View

Vasilopoulou M, Douvas A, Georgiadou D, Palilis L, Kennou S, Sygellou L . The influence of hydrogenation and oxygen vacancies on molybdenum oxides work function and gap states for application in organic optoelectronics. J Am Chem Soc. 2012; 134(39):16178-87. DOI: 10.1021/ja3026906. View

Alsaif M, Latham K, Field M, Yao D, Medhekar N, Medehkar N . Tunable plasmon resonances in two-dimensional molybdenum oxide nanoflakes. Adv Mater. 2014; 26(23):3931-7. DOI: 10.1002/adma.201306097. View

Wendumu T, Seifert G, Lorenz T, Joswig J, Enyashin A . Optical Properties of Triangular Molybdenum Disulfide Nanoflakes. J Phys Chem Lett. 2015; 5(21):3636-40. DOI: 10.1021/jz501604j. View

Morris G, Huey R, Lindstrom W, Sanner M, Belew R, Goodsell D . AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility. J Comput Chem. 2009; 30(16):2785-91. PMC: 2760638. DOI: 10.1002/jcc.21256. View

Liu Y, Yuan M, Qiao L, Guo R . An efficient colorimetric biosensor for glucose based on peroxidase-like protein-Fe3O4 and glucose oxidase nanocomposites. Biosens Bioelectron. 2013; 52:391-6. DOI: 10.1016/j.bios.2013.09.020. View

Li R, Zhen M, Guan M, Chen D, Zhang G, Ge J . A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C60-carboxyfullerenes. Biosens Bioelectron. 2013; 47:502-7. DOI: 10.1016/j.bios.2013.03.057. View

Pickup J, Hussain F, Evans N, Rolinski O, Birch D . Fluorescence-based glucose sensors. Biosens Bioelectron. 2005; 20(12):2555-65. DOI: 10.1016/j.bios.2004.10.002. View

10.

Hang D, Sharma K, Chen C, Islam S . Enhanced Photocatalytic Performance of ZnO Nanorods Coupled by Two-Dimensional α-MoO3 Nanoflakes under UV and Visible Light Irradiation. Chemistry. 2016; 22(36):12777-84. DOI: 10.1002/chem.201602141. View

11.

Gao Y, Wu Y, Di J . Colorimetric detection of glucose based on gold nanoparticles coupled with silver nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc. 2016; 173:207-212. DOI: 10.1016/j.saa.2016.09.023. View

12.

Amusat N, Beaupre L, Jhangri G, Pohar S, Simpson S, Warren S . Diabetes that impacts on routine activities predicts slower recovery after total knee arthroplasty: an observational study. J Physiother. 2014; 60(4):217-23. DOI: 10.1016/j.jphys.2014.09.006. View

13.

Marshall S, Flyvbjerg A . Prevention and early detection of vascular complications of diabetes. BMJ. 2006; 333(7566):475-80. PMC: 1557968. DOI: 10.1136/bmj.38922.650521.80. View

14.

Buhlmann P, Pretsch E, Bakker E . Carrier-Based Ion-Selective Electrodes and Bulk Optodes. 2. Ionophores for Potentiometric and Optical Sensors. Chem Rev. 2002; 98(4):1593-1688. DOI: 10.1021/cr970113+. View

15.

Wei H, Wang E . Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection. Anal Chem. 2008; 80(6):2250-4. DOI: 10.1021/ac702203f. View

16.

Harris M, Eastman R . Early detection of undiagnosed diabetes mellitus: a US perspective. Diabetes Metab Res Rev. 2000; 16(4):230-6. DOI: 10.1002/1520-7560(2000)9999:9999<::aid-dmrr122>3.0.co;2-w. View

17.

Dong Z, Lu L, Ko C, Yang C, Li S, Lee M . A MnO nanosheet-assisted GSH detection platform using an iridium(iii) complex as a switch-on luminescent probe. Nanoscale. 2017; 9(14):4677-4682. DOI: 10.1039/c6nr08357a. View

18.

Hu L, Yuan Y, Zhang L, Zhao J, Majeed S, Xu G . Copper nanoclusters as peroxidase mimetics and their applications to H2O2 and glucose detection. Anal Chim Acta. 2013; 762:83-6. DOI: 10.1016/j.aca.2012.11.056. View

19.

Yang C, Shang D, Liu N, Shi G, Shen X, Yu R . A Synaptic Transistor based on Quasi-2D Molybdenum Oxide. Adv Mater. 2017; 29(27). DOI: 10.1002/adma.201700906. View

20.

Xing Z, Tian J, Asiri A, Qusti A, Al-Youbi A, Sun X . Two-dimensional hybrid mesoporous Fe2O3-graphene nanostructures: a highly active and reusable peroxidase mimetic toward rapid, highly sensitive optical detection of glucose. Biosens Bioelectron. 2013; 52:452-7. DOI: 10.1016/j.bios.2013.09.029. View