A Myeloperoxidase Precursor, Pro-myeloperoxidase, is Present in Human Plasma and Elevated in Cardiovascular Disease Patients

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 Mar 29

PMID 29590135

Citations 11

Authors

Irada S Khalilova

Nina Dickerhof

Tessa J Mocatta

Catriona J Bhagra

Dougal R McClean

Christian Obinger

Anthony J Kettle

Affiliations

Soon will be listed here.

Abstract

Myeloperoxidase (MPO)-derived oxidants have emerged as a key contributor to tissue damage in inflammatory conditions such as cardiovascular disease. Pro-myeloperoxidase (pro-MPO), an enzymatically active precursor of myeloperoxidase (MPO), is known to be secreted from cultured bone marrow and promyelocytic leukemia cells, but evidence for the presence of pro-MPO in circulation is lacking. In the present study, we used a LC-MS/MS in addition to immunoblot analyses to show that pro-MPO is present in human blood plasma. Furthermore, we found that pro-MPO was more frequently detected in plasma from patients with myocardial infarction compared to plasma from control donors. Our study suggests that in addition to mature MPO, circulating pro-MPO may cause oxidative modifications of proteins thereby contributing to cardiovascular disease.

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