The Right End of Transposable Bacteriophage D108 Contains a 520 Base Pair Protein-encoding Sequence Not Present in Bacteriophage Mu

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Aug 25

PMID 2957646

Citations 1

Authors

G B Szatmari

M Lapointe

M S DuBow

Affiliations

Soon will be listed here.

Abstract

We have cloned and characterized the right end terminal 796 bp of the transposable Mu-like bacteriophage D108. This region encompasses a 520 bp region of D108-specific sequences not present in phage Mu that contain an open reading frame encoding a 12 KDa protein. This protein can be visualized in vivo when the region is placed downstream from the strong lac UV5 promoter. The open reading frame can be expressed from the dam-regulated mod promoter (for modification of D108 DNA), yet also contains its own dam-independent promoter for expression that is detectable by northern blot analysis late in the D108 lytic cycle. Comparison of this region of D108 DNA with the corresponding region of Mu DNA suggests that a complex rearrangement has occurred at the phages' right ends during their evolution.

Citing Articles

Role of bacteriophage Mu C protein in activation of the mom gene promoter.

Bolker M, Wulczyn F, Kahmann R J Bacteriol. 1989; 171(4):2019-27.

PMID: 2522924 PMC: 209852. DOI: 10.1128/jb.171.4.2019-2027.1989.

References

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Lacks S, Greenberg B . Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol. 1977; 114(1):153-68. DOI: 10.1016/0022-2836(77)90289-3. View

Ljungquist E, Bukhari A . State of prophage Mu DNA upon induction. Proc Natl Acad Sci U S A. 1977; 74(8):3143-7. PMC: 431469. DOI: 10.1073/pnas.74.8.3143. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Hull R, Gill G, Curtiss 3rd R . Genetic characterization of Mu-like bacteriophage D108. J Virol. 1978; 27(3):513-8. PMC: 525838. DOI: 10.1128/JVI.27.3.513-518.1978. View

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

Neidhardt F, Wirth R, Smith M, Van Bogelen R . Selective synthesis of plasmid-coded proteins by Escherichia coli during recovery from chloramphenicol treatment. J Bacteriol. 1980; 143(1):535-7. PMC: 294285. DOI: 10.1128/jb.143.1.535-537.1980. View

Gill G, Hull R, Curtiss 3rd R . Mutator bacteriophage D108 and its DNA: an electron microscopic characterization. J Virol. 1981; 37(1):420-30. PMC: 171019. DOI: 10.1128/JVI.37.1.420-430.1981. View

10.

Kleckner N . Transposable elements in prokaryotes. Annu Rev Genet. 1981; 15:341-404. DOI: 10.1146/annurev.ge.15.120181.002013. View

11.

Plasterk R, Vrieling H, Van de Putte P . Transcription initiation of Mu mom depends on methylation of the promoter region and a phage-coded transactivator. Nature. 1983; 301(5898):344-7. DOI: 10.1038/301344a0. View

12.

Luk K, SZYBALSKI W . Tandem transcription-termination sites in the late rightward operon of bacteriophage lambda. Mol Gen Genet. 1983; 189(2):289-97. DOI: 10.1007/BF00337819. View

13.

Toussaint A, Faelen M, Desmet L, Allet B . The products of gene A of the related phages Mu and D108 differ in their specificities. Mol Gen Genet. 1983; 190(1):70-9. DOI: 10.1007/BF00330326. View

14.

HAWLEY D, McClure W . Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983; 11(8):2237-55. PMC: 325881. DOI: 10.1093/nar/11.8.2237. View

15.

Kuzio J, Kropinski A . O-antigen conversion in Pseudomonas aeruginosa PAO1 by bacteriophage D3. J Bacteriol. 1983; 155(1):203-12. PMC: 217670. DOI: 10.1128/jb.155.1.203-212.1983. View

16.

Plasterk R, Vollering M, Brinkman A, Van de Putte P . Analysis of the methylation-regulated Mu mom transcript. Cell. 1984; 36(1):189-96. DOI: 10.1016/0092-8674(84)90088-6. View

17.

Plasterk R, Van de Putte P . Genetic switches by DNA inversions in prokaryotes. Biochim Biophys Acta. 1984; 782(2):111-9. DOI: 10.1016/0167-4781(84)90013-7. View

18.

Craigie R, Mizuuchi M, Mizuuchi K . Site-specific recognition of the bacteriophage Mu ends by the Mu A protein. Cell. 1984; 39(2 Pt 1):387-94. DOI: 10.1016/0092-8674(84)90017-5. View

19.

Groenen M, Timmers E, Van de Putte P . DNA sequences at the ends of the genome of bacteriophage Mu essential for transposition. Proc Natl Acad Sci U S A. 1985; 82(7):2087-91. PMC: 397497. DOI: 10.1073/pnas.82.7.2087. View

20.

Tolias P, DuBow M . The cloning and characterization of the bacteriophage D108 regulatory DNA-binding protein ner. EMBO J. 1985; 4(11):3031-7. PMC: 554615. DOI: 10.1002/j.1460-2075.1985.tb04040.x. View