Development of a Cooperative Two-factor Adaptive-evolution Method to Enhance Lipid Production and Prevent Lipid Peroxidation in Sp

Overview

Journal Biotechnol Biofuels

Publisher Biomed Central

Specialty Biotechnology

Date 2018 Mar 23

PMID 29563968

Citations 22

Authors

Xiao-Man Sun

Lu-Jing Ren

Zhi-Qian Bi

Xiao-Jun Ji

Quan-Yu Zhao

Ling Jiang

He Huang

Affiliations

Soon will be listed here.

Abstract

Background: sp. is a marine microalga with great potential as a promising sustainable source of lipids rich in docosahexaenoic acid (DHA). This organism's lipid accumulation machinery can be induced by various stress conditions, but this stress induction usually comes at the expense of lower biomass in industrial fermentations. Moreover, oxidative damage induced by various environmental stresses can result in the peroxidation of lipids, and especially polyunsaturated fatty acids, which causes unstable DHA production, but is often ignored in fermentation processes. Therefore, it is urgent to develop new production strains that not only have a high DHA production capacity, but also possess strong antioxidant defenses.

Results: Adaptive laboratory evolution (ALE) is an effective method for the development of beneficial phenotypes in industrial microorganisms. Here, a novel cooperative two-factor ALE strategy based on concomitant low temperature and high salinity was applied to improve the production capacity of sp. Low-temperature conditions were used to improve the DHA content, and high salinity was applied to stimulate lipid accumulation and enhance the antioxidative defense systems of sp. After 30 adaptation cycles, a maximal cell dry weight of 126.4 g/L and DHA yield of 38.12 g/L were obtained in the endpoint strain ALE-TF30, which was 27.42 and 57.52% higher than parental strain, respectively. Moreover, the fact that ALE-TF30 had the lowest concentrations of reactive oxygen species and malondialdehyde among all strains indicated that lipid peroxidation was greatly suppressed by the evolutionary process. Accordingly, the ALE-TF30 strain exhibited an overall increase of gene expression levels of antioxidant enzymes and polyketide synthases compared to the parental strain.

Conclusion: This study provides important clues on how to overcome the negative effects of lipid peroxidation on DHA production in sp. Taken together, the cooperative two-factor ALE process can not only increase the accumulation of lipids rich in DHA, but also prevent the loss of produced lipid caused by lipid peroxidation. The strategy proposed here may provide a new and alternative direction for the industrial cultivation of oil-producing microalgae.

Citing Articles

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Yang X, Wei L, Liang S, Wang Z, Li S Mar Drugs. 2024; 22(8).

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The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics.

Cutolo E, Caferri R, Campitiello R, Cutolo M Mar Drugs. 2023; 21(12).

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Qin J, Kurt E, LBassi T, Sa L, Xie D Front Microbiol. 2023; 14:1280296.

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Development of Schizochytrium sp. strain HS01 with high-DHA and low-saturated fatty acids production by multi-pronged adaptive evolution.

Zhong H, Zhang M, Chen L, Liu W, Tao Y Biotechnol Lett. 2023; 45(9):1147-1157.

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Propanol and 1, 3-propanediol enhance fatty acid accumulation synergistically in ATCC 20888.

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