Agnoprotein Is an Essential Egress Factor During BK Polyomavirus Infection

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2018 Mar 23

PMID 29562663

Citations 18

Authors

Margarita-Maria Panou

Emma L Prescott

Daniel L Hurdiss

Gemma Swinscoe

Michael Hollinshead

Laura G Caller

Ethan L Morgan

Louisa Carlisle

Marietta Muller

Michelle Antoni

David Kealy

Neil A Ranson

Colin M Crump

Andrew MacDonald

Affiliations

Soon will be listed here.

Abstract

BK polyomavirus (BKPyV; hereafter referred to as BK) causes a lifelong chronic infection and is associated with debilitating disease in kidney transplant recipients. Despite its importance, aspects of the virus life cycle remain poorly understood. In addition to the structural proteins, the late region of the BK genome encodes for an auxiliary protein called agnoprotein. Studies on other polyomavirus agnoproteins have suggested that the protein may contribute to virion infectivity. Here, we demonstrate an essential role for agnoprotein in BK virus release. Viruses lacking agnoprotein fail to release from host cells and do not propagate to wild-type levels. Despite this, agnoprotein is not essential for virion infectivity or morphogenesis. Instead, agnoprotein expression correlates with nuclear egress of BK virions. We demonstrate that the agnoprotein binding partner α-soluble N-ethylmaleimide sensitive fusion (NSF) attachment protein (α-SNAP) is necessary for BK virion release, and siRNA knockdown of α-SNAP prevents nuclear release of wild-type BK virions. These data highlight a novel role for agnoprotein and begin to reveal the mechanism by which polyomaviruses leave an infected cell.

Citing Articles

Target-specific peptides for BK virus agnoprotein identified through phage display screening: advancing antiviral therapeutics.

Xing X, Han J, Wang K, Tian F, Jiang C, Liang W Sci Rep. 2025; 15(1):2718.

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Massive entry of BK Polyomavirus induces transient cytoplasmic vacuolization of human renal proximal tubule epithelial cells.

Lorentzen E, Henriksen S, Rinaldo C PLoS Pathog. 2024; 20(11):e1012681.

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Single-cell RNA-sequencing of BK polyomavirus replication in primary human renal proximal tubular epithelial cells identifies specific transcriptome signatures and a novel mitochondrial stress pattern.

Weissbach F, Follonier O, Schmid S, Leuzinger K, Schmid M, Hirsch H J Virol. 2024; 98(12):e0138224.

PMID: 39513696 PMC: 11657676. DOI: 10.1128/jvi.01382-24.

Mouse polyomavirus infection induces lamin reorganisation.

Brustikova K, Ryabchenko B, Zackova S, Sroller V, Forstova J, Hornikova L FEBS J. 2024; 291(23):5133-5155.

PMID: 39288210 PMC: 11616003. DOI: 10.1111/febs.17275.

BK polyomavirus: latency, reactivation, diseases and tumorigenesis.

Zhou X, Zhu C, Li H Front Cell Infect Microbiol. 2023; 13:1263983.

PMID: 37771695 PMC: 10525381. DOI: 10.3389/fcimb.2023.1263983.

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