Immunohistochemical Study of Atrial Natriuretic Polypeptides in the Embryonic, Fetal and Neonatal Rat Heart

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1987 Jun 1

PMID 2955901

Citations 15

Authors

H Toshimori

K Toshimori

C Oura

H Matsuo

Affiliations

Soon will be listed here.

Abstract

An immunohistochemical study of atrial natriuretic polypeptides was carried out on embryonic, fetal and neonatal rat hearts, using an antiserum raised against alpha-human atrial natriuretic polypeptide (alpha-hANP). Weakly immunoreactive cells were seen in both atrial and ventricular walls at 11 days post coitum (pc). After this stage, the immunoreactive cells became more intensely stained in both atrial and ventricular walls. The immunoreactivity during the prenatal period was stronger in the superficial cell layer beneath the endocardium, than in the deep cell layer of the atrial wall. The cells in the trabecular meshwork also had an apparent, but weak, immunoreactivity, which showed a greater intensity in the left ventricle than in the right one. It is suggested that these immunoreactive cells in the ventricle may differentiate, in situ, into the cells of the impulse-conducting system during the further development of the heart.

Citing Articles

Characterizing the role of atrial natriuretic peptide signaling in the development of embryonic ventricular conduction system.

Govindapillai A, Hotchkiss A, Baguma-Nibasheka M, Rose R, Miquerol L, Smithies O Sci Rep. 2018; 8(1):6939.

PMID: 29720615 PMC: 5932026. DOI: 10.1038/s41598-018-25292-0.

Brain natriuretic peptide and P wave duration in dialysis patients.

Taskapan M, Senel S, Ulutas O, Aksoy Y, Sahin I, Kosar F Int Urol Nephrol. 2007; 39(2):603-8.

PMID: 17457688 DOI: 10.1007/s11255-007-9191-0.

Developmental anatomy of HNK-1 immunoreactivity in the embryonic rat heart: co-distribution with early conduction tissue.

Nakagawa M, Thompson R, Terracio L, Borg T Anat Embryol (Berl). 1993; 187(5):445-60.

PMID: 7688194 DOI: 10.1007/BF00174420.

Development of immunoreactive atrial natriuretic peptide in fetal hearts of spontaneously hypertensive and Wistar-Kyoto rats.

Scott J, Jennes L Anat Embryol (Berl). 1988; 178(4):359-63.

PMID: 2972230 DOI: 10.1007/BF00698667.

Atrial-specific granules in the hearts of normal and water-deprived rats.

Toshimori H, Toshimori K, Matsukura S, Oura C, Matsuo H Cell Tissue Res. 1988; 253(3):547-52.

PMID: 2846179 DOI: 10.1007/BF00219745.

References

de Bold A . Atrial natriuretic factor of the rat heart. Studies on isolation and properties. Proc Soc Exp Biol Med. 1982; 170(2):133-8. DOI: 10.3181/00379727-170-41408. View

BENCOSME S, Berger J . Specific granules in mammalian and non-mammalian vertebrate cardiocytes. Methods Achiev Exp Pathol. 1971; 5:173-213. View

Saper C, Standaert D, Currie M, Schwartz D, GELLER D, NEEDLEMAN P . Atriopeptin-immunoreactive neurons in the brain: presence in cardiovascular regulatory areas. Science. 1985; 227(4690):1047-9. DOI: 10.1126/science.2858127. View

Hsu S, RAINE L, FANGER H . Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-80. DOI: 10.1177/29.4.6166661. View

de Bold A . Tissue fractionation studies on the relationship between an atrial natriuretic factor and specific atrial granules. Can J Physiol Pharmacol. 1982; 60(3):324-30. DOI: 10.1139/y82-045. View

Forsgren S, Thornell L . The development of Purkinje fibres and ordinary myocytes in the bovine fetal heart. An ultrastructural study. Anat Embryol (Berl). 1981; 162(2):127-36. DOI: 10.1007/BF00306485. View

Kangawa K, Fukuda A, Matsuo H . Structural identification of beta- and gamma-human atrial natriuretic polypeptides. Nature. 1985; 313(6001):397-400. DOI: 10.1038/313397a0. View

Cantin M, Gutkowska J, Thibault G, Milne R, Ledoux S, MinLi S . Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands. Histochemistry. 1984; 80(2):113-27. DOI: 10.1007/BF00679984. View

de Bold A . Morphometric assessment of granulation in rat atrial cardiocytes: effect of age. J Mol Cell Cardiol. 1978; 10(8):717-24. DOI: 10.1016/0022-2828(78)90406-6. View

10.

Field E . The development of the conducting system in the heart of sheep. Br Heart J. 1951; 13(2):129-47. PMC: 479401. DOI: 10.1136/hrt.13.2.129. View

11.

Miyata A, Kangawa K, Toshimori T, Hatoh T, Matsuo H . Molecular forms of atrial natriuretic polypeptides in mammalian tissues and plasma. Biochem Biophys Res Commun. 1985; 129(1):248-55. DOI: 10.1016/0006-291x(85)91429-9. View

12.

Kawata M, Ueda S, Nakao K, MORII N, Kiso Y, Imura H . Immuohistochemical demonstration of alpha-atrial natriretic plypeptide-containing neurons in the rat brain. Histochemistry. 1985; 83(1):1-3. DOI: 10.1007/BF00495293. View

13.

KISCH B . Further studies about the microbodies in the atria of the heart. Exp Med Surg. 1965; 23(1):1-12. View

14.

Metz J, Mutt V, Forssmann W . Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria. Anat Embryol (Berl). 1984; 170(2):123-7. DOI: 10.1007/BF00318996. View

15.

Kangawa K, Matsuo H . Purification and complete amino acid sequence of alpha-human atrial natriuretic polypeptide (alpha-hANP). Biochem Biophys Res Commun. 1984; 118(1):131-9. DOI: 10.1016/0006-291x(84)91077-5. View

16.

Berger J, Rona G . Functional and fine structural heterogeneity of atrial cardiocytes. Methods Achiev Exp Pathol. 1971; 5:540-90. View

17.

VIRAGH S, CHALLICE C . The development of the early atrioventricular conduction system in the embryonic heart. Can J Physiol Pharmacol. 1983; 61(8):775-92. DOI: 10.1139/y83-121. View

18.

Jacobowitz D, Skofitsch G, KEISER H, Eskay R, Zamir N . Evidence for the existence of atrial natriuretic factor-containing neurons in the rat brain. Neuroendocrinology. 1985; 40(1):92-4. DOI: 10.1159/000124058. View

19.

Guesdon J, Ternynck T, Avrameas S . The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-9. DOI: 10.1177/27.8.90074. View

20.

JAMIESON J, Palade G . SPECIFIC GRANULES IN ATRIAL MUSCLE CELLS. J Cell Biol. 1964; 23:151-72. PMC: 2106511. DOI: 10.1083/jcb.23.1.151. View