Chemical Modification of Sarcoplasmic Reticulum with Methylbenzimidate. Stimulation of Ca2+ Efflux

Overview

Journal Biochem J

Specialty Biochemistry

Date 1987 Apr 1

PMID 2955781

Citations 4

Authors

V Shoshan-Barmatz

Affiliations

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Abstract

Treatment of sarcoplasmic reticulum membranes with 12 mM-methylbenzimidate (MBI) for 5 min, in the presence of 5 mM-ATP at pH 8.5, resulted in a 2-3-fold stimulation of ATP hydrolysis and over 90% inhibition of Ca2+ accumulation. This phenomenon was strictly dependent upon the presence of nucleotides with the following order of effectiveness: adenosine 5'-[beta, gamma-imido]triphosphate greater than or equal to ATP greater than UTP greater than ADP greater than AMP. Divalent cations such as Ca2+, Mg2+ and Mn2+, when present during the MBI treatment, prevented both the stimulation of ATPase activity and the inhibition of Ca2+ accumulation. Modification with MBI had no effect on E-P formation from ATP, ADP-ATP exchange, Ca2+ binding or ATP-Pi exchange catalysed by the membranes. Membranes modified with MBI in the presence of ATP and then passively loaded with Ca2+ released about 80% of their Ca2+ content within 3 s. Control membranes released only 3% of their Ca2+ during the same time period. MBI modification inhibited Ca2+ accumulation by proteoliposomes reconstituted with the partially purified ATPase but not with the purified ATPase fraction. These results suggest that MBI in the presence of ATP stimulates Ca2+ release by modifying a protein factor(s) other than the (Ca2+ + Mg2+)-ATPase.

Citing Articles

Stimulation of Ca2+ efflux from sarcoplasmic reticulum by preincubation with ATP and inorganic phosphate.

Shoshan-Barmatz V Biochem J. 1987; 247(3):497-504.

PMID: 2962569 PMC: 1148441. DOI: 10.1042/bj2470497.

Activation of Ca2+ release in isolated sarcoplasmic reticulum.

Shoshan-Barmatz V J Membr Biol. 1988; 103(1):67-77.

PMID: 2846845 DOI: 10.1007/BF01871933.

Pharmacology of calcium release from sarcoplasmic reticulum.

Palade P, Dettbarn C, Brunder D, Stein P, Hals G J Bioenerg Biomembr. 1989; 21(2):295-320.

PMID: 2546933 DOI: 10.1007/BF00812074.

Involvement of protein phosphorylation in activation of Ca2+ efflux from sarcoplasmic reticulum.

Gechtman Z, ORR I, Shoshan-Barmatz V Biochem J. 1991; 276 ( Pt 1):97-102.

PMID: 2039485 PMC: 1151148. DOI: 10.1042/bj2760097.

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