Single-cell Transcriptomics Reveals a New Dynamical Function of Transcription Factors During Embryonic Hematopoiesis

Overview

Journal Elife

Specialty Biology

Date 2018 Mar 21

PMID 29555020

Citations 22

Authors

Isabelle Bergiers

Tallulah Andrews

Ozge Vargel Bolukbasi

Andreas Buness

Ewa Janosz

Natalia Lopez-Anguita

Kerstin Ganter

Kinga Kosim

Cemre Celen

Gulce Itir Percin

Paul Collier

Bianka Baying

Vladimir Benes

Martin Hemberg

Christophe Lancrin

Affiliations

Soon will be listed here.

Abstract

Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell transcriptome assays. We found that a heptad of transcription factors (Runx1, Gata2, Tal1, Fli1, Lyl1, Erg and Lmo2) is specifically co-expressed in an intermediate population expressing both endothelial and hematopoietic markers. Within the heptad, we identified two sets of factors of opposing functions: one (Erg/Fli1) promoting the endothelial cell fate, the other (Runx1/Gata2) promoting the hematopoietic fate. Surprisingly, our data suggest that even though Fli1 initially supports the endothelial cell fate, it acquires a pro-hematopoietic role when co-expressed with Runx1. This work demonstrates the power of single-cell RNA-sequencing for characterizing complex transcription factor dynamics.

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