Design of FRET Probes for SNP RS1006737, Related to Mood Disorder

Overview

Journal Clin Pract Epidemiol Ment Health

Publisher Bentham Open

Specialty Psychiatry

Date 2018 Mar 16

PMID 29541152

Citations 5

Authors

Germano Orru

Mauro Giovanni Carta

Alessia Bramanti

Affiliations

Soon will be listed here.

Abstract

Background: Several studies have shown that the Single Nucleotide Polymorphism (SNP) in the CACAN1C gene, rs1006737, is related to different mood disorder illnesses, such as bipolar disorder and schizophrenia. Current day molecular procedures for allele detection of this gene can be very expensive and time consuming. Hence, a sensitive and specific molecular procedure for detecting these mutations in a large number of subjects is desirable, especially for research groups who have no complex laboratory equipment.

Objective: The possibility of using a Fluorescence Resonance Energy Transfer (FRET) probe was evaluated by means of bioinformatic tools, designed for forecasting the molecular behavior of DNA probes used in the research field or for laboratory analysis methods.

Method: In this study we used the DINAMelt Web Server to predict the ms of FRET oligo in the presence of the A and/or G allele in rs1006737. The PCR primers were designed by using oligo 4 and oligo 6 primer analysis software.

Results: The molecular probe described in this study detected a m difference of 5-6°C between alleles A and G in rs1006737, which also showed good discrimination for a heterozygous profile for this genomic region.

Conclusion: Although studies represent a relatively new avenue of inquiry, they have now started to be used to predict how a molecular probe interacts with its biological target, reducing the time and costs of molecular test tuning. The results of this study seem promising for further laboratory tests on allele detection in rs1006737 region.

Citing Articles

Is a Genetic Variant associated with Bipolar Disorder Frequent in People without Bipolar Disorder but with Characteristics of Hyperactivity and Novelty Seeking?.

Kalcev G, Scano A, Orru G, Primavera D, Cossu G, Nardi A Clin Pract Epidemiol Ment Health. 2023; 19:e174501792303280.

PMID: 37916199 PMC: 10351339. DOI: 10.2174/17450179-v19-e230419-2022-53.

Usefulness of salivary sampling for the molecular detection of a genetic variant associated with bipolar disorders.

Scano A, Kalcev G, Piras M, Fais S, Cossu G, Gonzalez C J Public Health Res. 2023; 12(1):22799036221146915.

PMID: 36967730 PMC: 10037733. DOI: 10.1177/22799036221146915.

Is Bipolar Disorder the Consequence of a Genetic Weakness or Not Having Correctly Used a Potential Adaptive Condition?.

Carta M, Kalcev G, Scano A, Primavera D, Orru G, Gureye O Brain Sci. 2023; 13(1).

PMID: 36671999 PMC: 9856125. DOI: 10.3390/brainsci13010016.

Can an Investigation of a Single Gene be Effective in Differentiating Certain Features of the Bipolar Disorder Profile?.

Piras M, Scano A, Orru G, Preti A, Marchese C, Kalcev G Clin Pract Epidemiol Ment Health. 2022; 17:187-189.

PMID: 35173786 PMC: 8728559. DOI: 10.2174/1745017902117010187.

The COVID-19 incidence in Italian regions correlates with low temperature, mobility and PM10 pollution but lethality only with low temperature.

Carta M, Minerba L, Demontis R, Orru G, Romano F, Scano A J Public Health Res. 2021; 10(4).

PMID: 34121380 PMC: 8636947. DOI: 10.4081/jphr.2021.2303.

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