Wharton's Jelly Derived Mesenchymal Stem Cells: Comparing Human and Horse

Overview

Journal Stem Cell Rev Rep

Publisher Springer

Specialty Cell Biology

Date 2018 Mar 7

PMID 29508214

Citations 8

Authors

Barbara Merlo

Gabriella Teti

Eleonora Mazzotti

Laura Ingra

Viviana Salvatore

Marina Buzzi

Giorgia Cerqueni

Manuela Dicarlo

Aliai Lanci

Carolina Castagnetti

Eleonora Iacono

Affiliations

Soon will be listed here.

Abstract

Wharton's jelly (WJ) is an important source of mesenchymal stem cells (MSCs) both in human and other animals. The aim of this study was to compare human and equine WJMSCs. Human and equine WJMSCs were isolated and cultured using the same protocols and culture media. Cells were characterized by analysing morphology, growth rate, migration and adhesion capability, immunophenotype, differentiation potential and ultrastructure. Results showed that human and equine WJMSCs have similar ultrastructural details connected with intense synthetic and metabolic activity, but differ in growth, migration, adhesion capability and differentiation potential. In fact, at the scratch assay and transwell migration assay, the migration ability of human WJMSCs was higher (P < 0.05) than that of equine cells, while the volume of spheroids obtained after 48 h of culture in hanging drop was larger than the volume of equine ones (P < 0.05), demonstrating a lower cell adhesion ability. This can also revealed in the lower doubling time of equine cells (3.5 ± 2.4 days) as compared to human (6.5 ± 4.3 days) (P < 0.05), and subsequently in the higher number of cell doubling after 44 days of culture observed for the equine (20.3 ± 1.7) as compared to human cells (8.7 ± 2.4) (P < 0.05), and to the higher (P < 0.05) ability to form fibroblast colonies at P3. Even if in both species tri-lineage differentiation was achieved, equine cells showed an higher chondrogenic and osteogenic differentiation ability (P < 0.05). Our findings indicate that, although the ultrastructure demonstrated a staminal phenotype in human and equine WJMSCs, they showed different properties reflecting the different sources of MSCs.

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References

De Schauwer C, Meyer E, Van de Walle G, Van Soom A . Markers of stemness in equine mesenchymal stem cells: a plea for uniformity. Theriogenology. 2011; 75(8):1431-43. DOI: 10.1016/j.theriogenology.2010.11.008. View

Vangsness Jr C, Sternberg H, Harris L . Umbilical Cord Tissue Offers the Greatest Number of Harvestable Mesenchymal Stem Cells for Research and Clinical Application: A Literature Review of Different Harvest Sites. Arthroscopy. 2015; 31(9):1836-43. DOI: 10.1016/j.arthro.2015.03.014. View

Pasquinelli G, Tazzari P, Ricci F, Vaselli C, Buzzi M, Conte R . Ultrastructural characteristics of human mesenchymal stromal (stem) cells derived from bone marrow and term placenta. Ultrastruct Pathol. 2007; 31(1):23-31. DOI: 10.1080/01913120601169477. View

Troyer D, Weiss M . Wharton's jelly-derived cells are a primitive stromal cell population. Stem Cells. 2007; 26(3):591-9. PMC: 3311226. DOI: 10.1634/stemcells.2007-0439. View

Li G, Zhang X, Wang H, Wang X, Meng C, Chan C . Comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord, and placenta: implication in the migration. Proteomics. 2009; 9(1):20-30. DOI: 10.1002/pmic.200701195. View

Janderova L, McNeil M, Murrell A, Mynatt R, Smith S . Human mesenchymal stem cells as an in vitro model for human adipogenesis. Obes Res. 2003; 11(1):65-74. DOI: 10.1038/oby.2003.11. View

Patterson-Kane J, Becker D, Rich T . The pathogenesis of tendon microdamage in athletes: the horse as a natural model for basic cellular research. J Comp Pathol. 2012; 147(2-3):227-47. DOI: 10.1016/j.jcpa.2012.05.010. View

Orsolic I, Jurada D, Pullen N, Oren M, Eliopoulos A, Volarevic S . The relationship between the nucleolus and cancer: Current evidence and emerging paradigms. Semin Cancer Biol. 2016; 37-38:36-50. DOI: 10.1016/j.semcancer.2015.12.004. View

Pascucci L, Mercati F, Marini C, Ceccarelli P, DallAglio C, Pedini V . Ultrastructural morphology of equine adipose-derived mesenchymal stem cells. Histol Histopathol. 2010; 25(10):1277-85. DOI: 10.14670/HH-25.1277. View

10.

Rainaldi G, Pinto B, Piras A, Vatteroni L, Simi S, Citti L . Reduction of proliferative heterogeneity of CHEF18 Chinese hamster cell line during the progression toward tumorigenicity. In Vitro Cell Dev Biol. 1991; 27A(12):949-52. DOI: 10.1007/BF02631122. View

11.

Suzuki S, Muneta T, Tsuji K, Ichinose S, Makino H, Umezawa A . Properties and usefulness of aggregates of synovial mesenchymal stem cells as a source for cartilage regeneration. Arthritis Res Ther. 2012; 14(3):R136. PMC: 3446519. DOI: 10.1186/ar3869. View

12.

Crovace A, Lacitignola L, Rossi G, Francioso E . Histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in collagenase-induced tendinitis of equine superficial digital flexor tendon. Vet Med Int. 2010; 2010:250978. PMC: 2859019. DOI: 10.4061/2010/250978. View

13.

Smith R . Mesenchymal stem cell therapy for equine tendinopathy. Disabil Rehabil. 2008; 30(20-22):1752-8. DOI: 10.1080/09638280701788241. View

14.

Iacono E, Rossi B, Merlo B . Stem cells from foetal adnexa and fluid in domestic animals: an update on their features and clinical application. Reprod Domest Anim. 2015; 50(3):353-64. DOI: 10.1111/rda.12499. View

15.

Wang W, Itaka K, Ohba S, Nishiyama N, Chung U, Yamasaki Y . 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials. 2009; 30(14):2705-15. DOI: 10.1016/j.biomaterials.2009.01.030. View

16.

De Schauwer C, Piepers S, Van de Walle G, Demeyere K, Hoogewijs M, Govaere J . In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 2012; 81(4):312-23. DOI: 10.1002/cyto.a.22026. View

17.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D . Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315-7. DOI: 10.1080/14653240600855905. View

18.

Harding J, Roberts R, Mirochnitchenko O . Large animal models for stem cell therapy. Stem Cell Res Ther. 2013; 4(2):23. PMC: 3706788. DOI: 10.1186/scrt171. View

19.

Ibrahim S, Steinbach F . Immunoprecipitation of equine CD molecules using anti-human MABs previously analyzed by flow cytometry and immunohistochemistry. Vet Immunol Immunopathol. 2011; 145(1-2):7-13. DOI: 10.1016/j.vetimm.2011.07.021. View

20.

Piccinini F, Tesei A, Arienti C, Bevilacqua A . Cancer multicellular spheroids: volume assessment from a single 2D projection. Comput Methods Programs Biomed. 2015; 118(2):95-106. DOI: 10.1016/j.cmpb.2014.12.003. View