Human IPSC-derived Mesenchymal Stem Cells Encapsulated in PEGDA Hydrogels Mature into Valve Interstitial-like Cells

Overview

Journal Acta Biomater

Publisher Elsevier

Specialty Biomedical Engineering

Date 2018 Mar 6

PMID 29505894

Citations 26

Authors

Aline L Y Nachlas

Siyi Li

Rajneesh Jha

Monalisa Singh

Chunhui Xu

Michael E Davis

Affiliations

Soon will be listed here.

Abstract

Statement Of Significance: Developing a suitable cell source is a critical component for the success and durability of tissue engineered heart valves. The significance of this study is the generation of iPSCs-derived mesenchymal stem cells (iMSCs) that have the capacity to mature into valve interstitial-like cells when introduced into a 3D cell culture designed to mimic the layers of the valve leaflet. iMSCs were generated using a feeder-free protocol, which is one major advantage over other methods, as it is more clinically relevant. In addition to generating a potential new cell source for heart valve tissue engineering, this study also highlights the importance of a 3D culture environment to influence cell phenotype and function.

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References

Zhou Y, Zeng F . Integration-free methods for generating induced pluripotent stem cells. Genomics Proteomics Bioinformatics. 2013; 11(5):284-7. PMC: 4357834. DOI: 10.1016/j.gpb.2013.09.008. View

Xu C, Inokuma M, Denham J, Golds K, Kundu P, Gold J . Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol. 2001; 19(10):971-4. DOI: 10.1038/nbt1001-971. View

Hinton R, Yutzey K . Heart valve structure and function in development and disease. Annu Rev Physiol. 2010; 73:29-46. PMC: 4209403. DOI: 10.1146/annurev-physiol-012110-142145. View

Alsoufi B . Aortic valve replacement in children: Options and outcomes. J Saudi Heart Assoc. 2014; 26(1):33-41. PMC: 3936244. DOI: 10.1016/j.jsha.2013.11.003. View

Yang F, Williams C, Wang D, Lee H, Manson P, Elisseeff J . The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells. Biomaterials. 2005; 26(30):5991-8. DOI: 10.1016/j.biomaterials.2005.03.018. View

Mabry K, Lawrence R, Anseth K . Dynamic stiffening of poly(ethylene glycol)-based hydrogels to direct valvular interstitial cell phenotype in a three-dimensional environment. Biomaterials. 2015; 49:47-56. PMC: 4346780. DOI: 10.1016/j.biomaterials.2015.01.047. View

Takkenberg J, Rajamannan N, Rosenhek R, Kumar A, Carapetis J, Yacoub M . The need for a global perspective on heart valve disease epidemiology. The SHVD working group on epidemiology of heart valve disease founding statement. J Heart Valve Dis. 2008; 17(1):135-9. View

Ali S, Cuchiara M, West J . Micropatterning of poly(ethylene glycol) diacrylate hydrogels. Methods Cell Biol. 2014; 121:105-19. DOI: 10.1016/B978-0-12-800281-0.00008-7. View

Hinderer S, Seifert J, Votteler M, Shen N, Rheinlaender J, Schaffer T . Engineering of a bio-functionalized hybrid off-the-shelf heart valve. Biomaterials. 2013; 35(7):2130-9. DOI: 10.1016/j.biomaterials.2013.10.080. View

10.

Okita K, Ichisaka T, Yamanaka S . Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448(7151):313-7. DOI: 10.1038/nature05934. View

11.

Taylor P, Batten P, Brand N, Thomas P, Yacoub M . The cardiac valve interstitial cell. Int J Biochem Cell Biol. 2002; 35(2):113-8. DOI: 10.1016/s1357-2725(02)00100-0. View

12.

Huang W, Xiao D, Wang Y, Shan Z, Liu X, Lin Q . Fn14 promotes differentiation of human mesenchymal stem cells into heart valvular interstitial cells by phenotypic characterization. J Cell Physiol. 2013; 229(5):580-7. DOI: 10.1002/jcp.24480. View

13.

Hasan A, Ragaert K, Swieszkowski W, Selimovic S, Paul A, Camci-Unal G . Biomechanical properties of native and tissue engineered heart valve constructs. J Biomech. 2013; 47(9):1949-63. DOI: 10.1016/j.jbiomech.2013.09.023. View

14.

Bryant S, Nuttelman C, Anseth K . Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro. J Biomater Sci Polym Ed. 2000; 11(5):439-57. DOI: 10.1163/156856200743805. View

15.

Shinoka T, Ma P, Shum-Tim D, Breuer C, Cusick R, Zund G . Tissue-engineered heart valves. Autologous valve leaflet replacement study in a lamb model. Circulation. 1996; 94(9 Suppl):II164-8. View

16.

Kang R, Zhou Y, Tan S, Zhou G, Aagaard L, Xie L . Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity. Stem Cell Res Ther. 2015; 6:144. PMC: 4539932. DOI: 10.1186/s13287-015-0137-7. View

17.

Xu C, Jiang J, Sottile V, McWhir J, Lebkowski J, Carpenter M . Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth. Stem Cells. 2004; 22(6):972-80. DOI: 10.1634/stemcells.22-6-972. View

18.

Katsara O, Mahaira L, Iliopoulou E, Moustaki A, Antsaklis A, Loutradis D . Effects of donor age, gender, and in vitro cellular aging on the phenotypic, functional, and molecular characteristics of mouse bone marrow-derived mesenchymal stem cells. Stem Cells Dev. 2011; 20(9):1549-61. DOI: 10.1089/scd.2010.0280. View

19.

Weber B, Emmert M, Hoerstrup S . Stem cells for heart valve regeneration. Swiss Med Wkly. 2012; 142:w13622. DOI: 10.4414/smw.2012.13622. View

20.

Sacks M, Schoen F, Mayer J . Bioengineering challenges for heart valve tissue engineering. Annu Rev Biomed Eng. 2009; 11:289-313. DOI: 10.1146/annurev-bioeng-061008-124903. View