Domain III of Cry1Ac Is Critical to Binding and Toxicity Against Soybean Looper (Chrysodeixis Includens) but Not to Velvetbean Caterpillar (Anticarsia Gemmatalis)

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2018 Mar 3

PMID 29495466

Citations 6

Authors

Rubina Mushtaq

Abdul Rauf Shakoori

Juan Luis Jurat-Fuentes

Affiliations

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Abstract

Insecticidal proteins Cry1Ac and Cry2Ac7 from the bacterium (Bt) belong to the three-domain family of Bt toxins. Commercial transgenic soybean hybrids produce Cry1Ac to control the larvae of the soybean looper () and the velvet bean caterpillar (). The specificity of Cry1Ac is determined by loops extending from domain II and regions of domain III in the three-dimensional structure of the toxin. In this study, we constructed a hybrid toxin (H1.2Ac) containing domains I and II of Cry1Ac and domain III of Cry2Ac7, in an attempt to obtain a protein with enhanced toxicity compared to parental toxins. Bioassays with H1.2Ac revealed toxicity against the larvae of but not against . Saturation binding assays with radiolabeled toxins and midgut brush border membrane vesicles demonstrated no specific H1.2Ac binding to , while binding in was specific and saturable. Results from competition binding assays supported the finding that Cry1Ac specificity against is mainly dictated by domain II. Taken together, these distinct interactions with binding sites may help explain the differential susceptibility to Cry1Ac in and , and guide the design of improved toxins against soybean pests.

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