Overexpression of HvIcy6 in Barley Enhances Resistance Against Tetranychus Urticae and Entails Partial Transcriptomic Reprogramming

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2018 Mar 2

PMID 29494488

Citations 9

Authors

M Estrella Santamaria

Mercedes Diaz-Mendoza

David Perez-Herguedas

Goetz Hensel

Jochen Kumlehn

Isabel Diaz

Manuel Martinez

Affiliations

Soon will be listed here.

Abstract

Cystatins have been largely used for pest control against phytophagous species. However, cystatins have not been commonly overexpressed in its cognate plant species to test their pesticide capacity. Since the inhibitory role of barley HvCPI-6 cystatin against the phytophagous mite has been previously demonstrated, the purpose of our study was to determine if barley transgenic lines overexpressing its own gene were more resistant against this phytophagous infestation. Besides, a transcriptomic analysis was done to find differential expressed genes among wild-type and transformed barley plants. Barley plants overexpressing cystatin gene remained less susceptible to attack when compared to wild-type plants, with a significant lesser foliar damaged area and a lower presence of the mite. Transcriptomic analysis revealed a certain reprogramming of cellular metabolism and a lower expression of several genes related to photosynthetic activity. Therefore, although caution should be taken to discard potential deleterious pleiotropic effects, cystatins may be used as transgenes with impact on agricultural crops by conferring enhanced levels of resistance to phytophagous pests.

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