Metabolic Characterization of Human Aqueous Humor in the Cataract Progression After Pars Plana Vitrectomy

Overview

Journal BMC Ophthalmol

Publisher Biomed Central

Specialty Ophthalmology

Date 2018 Mar 1

PMID 29486760

Citations 13

Authors

Yinghong Ji

Xianfang Rong

Yi Lu

Affiliations

Soon will be listed here.

Abstract

Background: While pars plana vitrectomy (PPV) has become the third most commonly performed surgery in the world, it can also induce multiple post complications easily. Among them, cataract progression is the most frequent one that can lead to blindness eventually.

Methods: To understand the underlying mechanisms of post PPV cataract progression, we performed comprehensive metabolic characterization of aqueous humor (AH) samples from 20 cataract patients (10 post PPV complication and 10 none PPV cataract) by a non-targeted metabolomic analysis using gas chromatography combined with time-of-flight mass spectrometer (GC/TOF MS).

Results: A total of 263 metabolites were identified and eight of them are determined to be significantly different (VIP ≥ 1 and p ≤ 0.05) between post PPV group and none PPV control group. The significantly changed metabolites included glutaric acid and pelargonic acid that play key roles in the regulation of oxidative stress and inflammatory responses. Furthermore, we constructed a metabolic regulatory network in each group based on metabolite-metabolite correlations, which reveals key metabolic pathways and regulatory elements including amino acids and lipids metabolisms that are related to cataract progression.

Conclusions: Altogether, this work discovered some potential metabolite biomarkers for post PPV cataract diagnostics, as well as casted some novel insights into the underlying mechanisms of cataract progression after PPV.

Citing Articles

Differential Signaling Pathways Identified in Aqueous Humor, Anterior Capsule, and Crystalline Lens of Age-Related, Diabetic, and Post-Vitrectomy Cataract.

Karakosta C, Samiotaki M, Bisoukis A, Bougioukas K, Panayotou G, Papaconstantinou D Proteomes. 2025; 13(1).

PMID: 39982317 PMC: 11843915. DOI: 10.3390/proteomes13010007.

Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization.

Wei Q, Yu Z, Zhou X, Gong R, Jiang R, Xu G Metabolites. 2023; 13(8).

PMID: 37623844 PMC: 10456621. DOI: 10.3390/metabo13080900.

The Interplay Between Metabolites and MicroRNAs in Aqueous Humor to Coordinate Corneal Endothelium Integrity.

Ueno M, Yoshii K, Yamashita T, Sonomura K, Asada K, Ito E Ophthalmol Sci. 2023; 3(3):100299.

PMID: 37125267 PMC: 10141542. DOI: 10.1016/j.xops.2023.100299.

The First Evidence on the Occurrence of Bisphenol Analogues in the Aqueous Humor of Patients Undergoing Cataract Surgery.

Flieger J, Sniegocki T, Dolar-Szczasny J, Zaluska W, Rejdak R J Clin Med. 2022; 11(21).

PMID: 36362630 PMC: 9655480. DOI: 10.3390/jcm11216402.

Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides.

Kunikata H, Tawarayama H, Tsuda S, Akaike T, Nakazawa T Sci Rep. 2022; 12(1):19243.

PMID: 36357454 PMC: 9649782. DOI: 10.1038/s41598-022-21677-4.

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