Tools for Studying Growth Patterns and Chemical Dynamics of Aggregated Exposed to Different Electron Acceptors in an Alginate Bead Model

Overview

Journal NPJ Biofilms Microbiomes

Date 2018 Feb 27

PMID 29479470

Citations 18

Authors

Majken Sonderholm

Klaus Koren

Daniel Wangpraseurt

Peter Ostrup Jensen

Mette Kolpen

Kasper Norskov Kragh

Thomas Bjarnsholt

Michael Kuhl

Affiliations

Soon will be listed here.

Abstract

In chronic infections, bacterial pathogens typically grow as small dense cell aggregates embedded in a matrix consisting of, e.g., wound bed sludge or lung mucus. Such biofilm growth mode exhibits extreme tolerance towards antibiotics and the immune defence system. The bacterial aggregates are exposed to physiological heterogeneity and O limitation due to steep chemical gradients through the matrix, which is are hypothesised to contribute to antibiotic tolerance. Using a novel combination of microsensor and bioimaging analysis, we investigated growth patterns and chemical dynamics of the pathogen in an alginate bead model, which mimics growth in chronic infections better than traditional biofilm experiments in flow chambers. Growth patterns were strongly affected by electron acceptor availability and the presence of chemical gradients, where the combined presence of O and nitrate yielded highest bacterial growth by combined aerobic respiration and denitrification.

Citing Articles

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Determining effects of nitrate, arginine, and ferrous on antibiotic recalcitrance of clinical strains of Pseudomonas aeruginosa in biofilm-inspired alginate encapsulates.

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Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays.

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