Immunocytochemistry of Magnocellular Neurons of Supraoptic and Paraventricular Nuclei of Normal and Brattleboro Rats with Vasopressin Anti-idiotype Antibody

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1986 Jan 1

PMID 2947686

Citations 3

Authors

K M Knigge

D T Piekut

D J Berlove

Affiliations

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Abstract

A vasopressin anti-idiotype antibody was generated by immunization with purified IgG of a primary vasopressin antiserum. The anti-idiotype antibody immunostained neurons in the supraoptic and paraventricular nuclei of the hypothalamus of normal and Brattleboro rats. The distribution of immunostained perikarya in these hypothalamic nuclei together with the staining of fibers in median eminence and neural lobe was similar to that observed in normal rats with anti-vasopressin and suggests strongly that vasopressinergic neurons are being stained. Absorption studies with vasopressin and a vasopressin-binding receptor protein further indicate that a receptor associated with vasopressinergic neurons is recognized by the anti-idiotype antibody.

Citing Articles

Neuropeptidomics of the supraoptic rat nucleus.

Bora A, Annangudi S, Millet L, Rubakhin S, Forbes A, Kelleher N J Proteome Res. 2008; 7(11):4992-5003.

PMID: 18816085 PMC: 2646869. DOI: 10.1021/pr800394e.

Anti-idiotypes used in immunohistochemistry.

Wang M Histochem J. 1990; 22(10):519-25.

PMID: 2289892 DOI: 10.1007/BF01005973.

Co-localization of putative vasopressin receptors and vasopressinergic neurons in rat hypothalamus.

Berlove D, Piekut D Histochemistry. 1990; 94(6):653-7.

PMID: 2149133 DOI: 10.1007/BF00271993.

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