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Perfusion Cuvette for the Simultaneous Measurement of Mechanical, Optical and Energetic Parameters of Skinned Muscle Fibres

Overview
Journal Pflugers Arch
Specialty Physiology
Date 1986 Nov 1
PMID 2947040
Citations 45
Authors
K Guth
R Wojciechowski
Affiliations
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Abstract

A micro cuvette is described, which allows to measure mechanical, optical and energetic parameters of single skinned muscle fibres or small fibre bundles. Two examples for possible applications are given: The measurement of ATPase activity of single muscle fibres. The detection of fluorescence changes due to Ca binding to fluorescence labeled TnC, which was incorporated into small bundles of rabbit psoas fibres before the experiment.

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References
1.
Ford L, HUXLEY A, Simmons R . Tension responses to sudden length change in stimulated frog muscle fibres near slack length. J Physiol. 1977; 269(2):441-515. PMC: 1283722. DOI: 10.1113/jphysiol.1977.sp011911. View
2.
Guth K, Kuhn H, Drexler B, Berberich W, Ruegg J . Stiffness and tension during and after sudden length changes of glycerinated single insect fibrillar muscle fibres. Biophys Struct Mech. 1979; 5(4):255-76. DOI: 10.1007/BF02426662. View
3.
Fabiato A, Fabiato F . Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris). 1979; 75(5):463-505. View
4.
Guth K . Polarization of tryptophan fluorescence measurements in muscle. A re-evaluation. Biophys Struct Mech. 1980; 6(2):81-93. DOI: 10.1007/BF00535746. View
5.
Griffiths P, Guth K, Kuhn H, Ruegg J . ATPase activity in rapidly activated skinned muscle fibres. Pflugers Arch. 1980; 387(2):167-73. DOI: 10.1007/BF00584268. View