Rapid Detection of Quantum Dot Immune Chromatography Nasopharyngeal Carcinoma EBNA1 Antibody

Overview

Journal Oncol Lett

Specialty Oncology

Date 2018 Feb 23

PMID 29467877

Authors

Jian He

Jing Xu

Ping Wu

Lingfeng Liao

Huatao Quan

Jing Kang

Yongquan Tian

Yaoyun Tang

Affiliations

Soon will be listed here.

Abstract

This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hydrochloride to generate quantum dot-labeled antigen. Gel imaging system showed that serum group and 60 patients with nasopharyngeal carcinoma had significant differences in 30 cases of normal adult serogroup brightness, Levene test in the two groups ELISA absorbance value was P<0.001, namely the nasopharyngeal carcinoma group and the normal adult serum group measured absorbance value difference was obvious. The statistical significance, and the detection technology were of high specificity and sensitivity. In conclusion, this study adopted double antigen clip combining immune chromatography test EBNA1 antibody in serum, compared with the traditional enzyme-linked immunoassays this method is more rapid, with simpler operation, rapid detection for developing quantum dot immune chromatography technology the EBNA1 antibody kit provides a theoretical basis.

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