Acute Exposure to Copper Induces Variable Intensity of Oxidative Stress in Goldfish Tissues

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2018 Feb 22

PMID 29464406

Citations 7

Authors

Viktor V Husak

Nadia M Mosiichuk

Olga I Kubrak

Tetiana M Matviishyn

Janet M Storey

Kenneth B Storey

Volodymyr I Lushchak

Affiliations

Soon will be listed here.

Abstract

Copper is an essential element, but at high concentrations, it is toxic for living organisms. The present study investigated the responses of goldfish, Carassius auratus, to 96 h exposure to 30, 300, or 700 μg L of copper II chloride (Cu). The content of protein carbonyls was higher in kidney (by 158%) after exposure to 700 mg L copper, whereas in gills, liver, and brain, we observed lower content of protein carbonyls after exposure to copper compared with control values. Exposure to copper resulted in increased levels of lipid peroxides in gills (76%) and liver (95-110%) after exposure to 300 and 700 μg L Cu. Low molecular mass thiols were depleted by 23-40% in liver and by 29-67% in kidney in response to copper treatment and can be used as biomarkers toxicity of copper. The activities of primary antioxidant enzymes, superoxide dismutase and catalase, were increased in liver as a result of Cu exposure, whereas in kidney catalase activity was decreased. The activities of glutathione-related enzymes, glutathione peroxidase, glutathione-S-transferase, and glutathione reductase were decreased as a result of copper exposure, but glutathione reductase activity increased by 25-40% in liver. Taken together, these data show that exposure of fish to Cu ions results in the development of low/high intensity oxidative stress reflected in enhanced activities of antioxidant and associated enzymes in different goldfish tissues.

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