Immunogenicity of Heparin-binding Hemagglutinin Expressed by GS115 Strain

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2018 Feb 20

PMID 29456820

Citations 1

Authors

Xindong Teng

Xiaoguang Chen

Ke Zhu

Hefei Xu

Affiliations

Soon will be listed here.

Abstract

Objectives: Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of () from the site of primary infection. Superior expression systems are required to obtain abundant proteins for the purpose of diagnosing infection or for the immunization. Here, HBHA was expressed by () GS115 strain , and the immunogenicity of HBHA was evaluated.

Materials And Methods: The gene of was cloned into the pPIC9K plasmid, which was good for electroporation into GS115 strain. Unlabeled HBHA protein was purified using a Sepharose CL-6B column, and its expression was confirmed using anti-HBHA polyclonal antibody from mouse serum. We injected C57BL/6 mice with HBHA/ dimethyldioctadecylammonium/trehalose 6,6'-dibehenate (HBHA/DDA/TDB) to investigate the immunogenicity of this potential vaccine.

Results: The results demonstrated that HBHA/DDA/TDB has the ability to induce high levels of HBHA-specific IgG antibody and its subclasses, as well as interferon-gamma, compared with injection of phosphate-buffered saline, DDA/TDB alone and Bacillus Calmette-Guérin (BCG) controls (<0.05). Moreover, the ratio of IgG2a/IgG1 of the HBHA/DDA/TDB group was higher than that of the BCG group (<0.05).

Conclusion: HBHA with no label has excellent immunogenicity, and is suitable for evaluating the effectiveness to prevent infection.

Citing Articles

Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins.

Karbalaei M, Rezaee S, Farsiani H J Cell Physiol. 2020; 235(9):5867-5881.

PMID: 32057111 PMC: 7228273. DOI: 10.1002/jcp.29583.

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