The Rice "fruit-weight 2.2-like" Gene Family Member OsFWL4 is Involved in the Translocation of Cadmium from Roots to Shoots

Overview

Journal Planta

Specialty Biology

Date 2018 Feb 18

PMID 29453663

Citations 7

Authors

Wentao Xiong

Peng Wang

Tianze Yan

Baobao Cao

Jun Xu

Defang Liu

Meizhong Luo

Affiliations

Soon will be listed here.

Abstract

Heterogeneous expression of the rice genes "fruit-weight 2.2-like" (OsFWL) affects Cd resistance in yeast, and OsFWL4 mediates the translocation of Cd from roots to shoots. Cadmium (Cd) induces chronic and toxic effects in humans. In a previous study (Xu et al. in Planta 238:643-655, 2013), we cloned the rice genes, designated OsFWL1-8, homologous to the tomato fruit-weight 2.2. Here, we show that expression of genes OsFWL3-7 in yeast confers resistance to Cd. The Cd contents of OsFWL3-, -4-, -6- and -7-transformed Cd(II)-sensitive yeast mutant ycf1 cells were strongly decreased compared with those of empty vector, with the strongest resistance to Cd observed in cells expressing OsFWL4. Evaluation of truncated and site-directed mutation derivatives revealed that the CCXXG motifs near the second transmembrane region of OsFWL4 are involved in Cd resistance in yeast. Real-time PCR analysis showed that OsFWL4 expression was induced by CdCl stress in rice seedlings. Compared with WT plants, the Cd contents in the shoots of RNAi mediated OsFWL4 knockdown plants were significantly decreased, and Cd translocation from roots to shoots was reduced. According to bimolecular fluorescence complementation, yeast two-hybrid and Western-blotting assays, the OsFWL4 protein forms homo-oligomers. These results suggest that OsFWL4 might act directly as a transporter and is involved in the translocation of Cd from roots to shoots in rice.

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References

Takahashi R, Ishimaru Y, Nakanishi H, K Nishizawa N . Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice. Plant Signal Behav. 2011; 6(11):1813-6. PMC: 3329356. DOI: 10.4161/psb.6.11.17587. View

Shimo H, Ishimaru Y, An G, Yamakawa T, Nakanishi H, K Nishizawa N . Low cadmium (LCD), a novel gene related to cadmium tolerance and accumulation in rice. J Exp Bot. 2011; 62(15):5727-34. PMC: 3223062. DOI: 10.1093/jxb/err300. View

Chen J, Yang L, Gu J, Bai X, Ren Y, Fan T . MAN3 gene regulates cadmium tolerance through the glutathione-dependent pathway in Arabidopsis thaliana. New Phytol. 2014; 205(2):570-82. DOI: 10.1111/nph.13101. View

Ishikawa S, Ishimaru Y, Igura M, Kuramata M, Abe T, Senoura T . Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice. Proc Natl Acad Sci U S A. 2012; 109(47):19166-71. PMC: 3511095. DOI: 10.1073/pnas.1211132109. View

Yuan L, Yang S, Liu B, Zhang M, Wu K . Molecular characterization of a rice metal tolerance protein, OsMTP1. Plant Cell Rep. 2011; 31(1):67-79. DOI: 10.1007/s00299-011-1140-9. View

Howden R, Andersen C, Goldsbrough P, Cobbett C . A cadmium-sensitive, glutathione-deficient mutant of Arabidopsis thaliana. Plant Physiol. 1995; 107(4):1067-73. PMC: 157238. DOI: 10.1104/pp.107.4.1067. View

Zimeri A, Dhankher O, McCaig B, Meagher R . The plant MT1 metallothioneins are stabilized by binding cadmiums and are required for cadmium tolerance and accumulation. Plant Mol Biol. 2005; 58(6):839-855. DOI: 10.1007/s11103-005-8268-3. View

Ha S, Smith A, Howden R, Dietrich W, Bugg S, Oconnell M . Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe. Plant Cell. 1999; 11(6):1153-64. PMC: 144235. DOI: 10.1105/tpc.11.6.1153. View

Honda R, Swaddiwudhipong W, Nishijo M, Mahasakpan P, Teeyakasem W, Ruangyuttikarn W . Cadmium induced renal dysfunction among residents of rice farming area downstream from a zinc-mineralized belt in Thailand. Toxicol Lett. 2010; 198(1):26-32. DOI: 10.1016/j.toxlet.2010.04.023. View

10.

Grill E, Winnacker E, Zenk M . Phytochelatins: the principal heavy-metal complexing peptides of higher plants. Science. 1985; 230(4726):674-6. DOI: 10.1126/science.230.4726.674. View

11.

Yamanaka T, Nakagawa Y, Mori K, Nakano M, Imamura T, Kataoka H . MCA1 and MCA2 that mediate Ca2+ uptake have distinct and overlapping roles in Arabidopsis. Plant Physiol. 2010; 152(3):1284-96. PMC: 2832256. DOI: 10.1104/pp.109.147371. View

12.

Lam S, Siu C, Hillmer S, Jang S, An G, Robinson D . Rice SCAMP1 defines clathrin-coated, trans-golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells. Plant Cell. 2007; 19(1):296-319. PMC: 1820953. DOI: 10.1105/tpc.106.045708. View

13.

Xu J, Xiong W, Cao B, Yan T, Luo T, Fan T . Molecular characterization and functional analysis of "fruit-weight 2.2-like" gene family in rice. Planta. 2013; 238(4):643-55. DOI: 10.1007/s00425-013-1916-y. View

14.

Wang Y, Zeng H, Zhou X, Huang F, Peng W, Liu L . Transformation of rice with large maize genomic DNA fragments containing high content repetitive sequences. Plant Cell Rep. 2015; 34(6):1049-61. DOI: 10.1007/s00299-015-1764-2. View

15.

Gazzarrini S, Kang M, Abenavoli A, Romani G, Olivari C, Gaslini D . Chlorella virus ATCV-1 encodes a functional potassium channel of 82 amino acids. Biochem J. 2009; 420(2):295-303. PMC: 2903877. DOI: 10.1042/BJ20090095. View

16.

Lanquar V, Lelievre F, Bolte S, Hames C, Alcon C, Neumann D . Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron. EMBO J. 2005; 24(23):4041-51. PMC: 1356305. DOI: 10.1038/sj.emboj.7600864. View

17.

Kobayashi T, K Nishizawa N . Iron sensors and signals in response to iron deficiency. Plant Sci. 2014; 224:36-43. DOI: 10.1016/j.plantsci.2014.04.002. View

18.

Voelker C, Schmidt D, Mueller-Roeber B, Czempinski K . Members of the Arabidopsis AtTPK/KCO family form homomeric vacuolar channels in planta. Plant J. 2006; 48(2):296-306. DOI: 10.1111/j.1365-313X.2006.02868.x. View

19.

Guo M, Rupe M, Dieter J, Zou J, Spielbauer D, Duncan K . Cell Number Regulator1 affects plant and organ size in maize: implications for crop yield enhancement and heterosis. Plant Cell. 2010; 22(4):1057-73. PMC: 2879740. DOI: 10.1105/tpc.109.073676. View

20.

Sasaki A, Yamaji N, Yokosho K, Ma J . Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice. Plant Cell. 2012; 24(5):2155-67. PMC: 3442593. DOI: 10.1105/tpc.112.096925. View